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Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.
PLOS ONE ( IF 2.9 ) Pub Date : 2021-07-26 , DOI: 10.1371/journal.pone.0255096
Noa Furth 1 , Shay Shilo 1 , Niv Cohen 1 , Nir Erez 1 , Vadim Fedyuk 1 , Alexander M Schrager 2 , Adina Weinberger 3 , Amiel A Dror 4, 5 , Asaf Zigron 5, 6 , Mona Shehadeh 5, 7 , Eyal Sela 4, 5 , Samer Srouji 5, 6 , Sharon Amit 8 , Itzchak Levy 8, 9 , Eran Segal 3 , Rony Dahan 10 , Dan Jones 11 , Daniel C Douek 2 , Efrat Shema 1
Affiliation  

The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.

中文翻译:

利用单分子技术对 COVID-19 进行基因和血清学检测的统一平台。

COVID-19 大流行提出了对多种诊断方法的需求,以快速检测病毒感染的不同阶段。单分子成像技术的灵活和定量特性使其成为开发新诊断工具的最佳选择。在此,我们提出了用于检测 SARS-CoV-2 的单分子无酶测定法的概念验证。我们开发的统一平台可以直接检测患者样本中的病毒遗传物质,以及由 IgG 和 IgM 抗体组成的免疫反应。因此,它建立了一个用于诊断 COVID-19 的平台,该平台也可以进行调整以诊断其他病原体。
更新日期:2021-07-26
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