Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans,Annals of Applied Biology

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Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-07-27 , DOI: 10.1111/aab.12721
Elizabeth Teixeira Almeida Ramos _{1,

2} , Carlos Henrique Salvino Gadelha Meneses ₃ , Marcia Soares Vidal ₂ , José Ivo Baldani ₂

Affiliation

Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 α-helices and 11 β-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 μg μl⁻¹). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. albilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.

中文翻译：

Gluconacin 的特性和作用模式，一种对白色黄单胞菌具有抗菌活性的细菌素

Gluconacin 是一种由Gluconacetobacter diazotrophicus PAL5产生的细菌素，此前已显示出对有益细菌和植物病原细菌的大范围抑制活性。本研究基于建模提出了葡糖酸的三维 (3D) 结构，并描述了该肽的一些理化特征和肽对植物病原细菌Xanthomonas albilineans ICMP196细胞的影响。Gluconacin 3D 结构模型结果表明有 7 个 α-螺旋和 11 个 β-折叠。指示微生物X. albilineans 的生长抑制发生在与葡糖酸(0.25 μg μl ^-1）。细菌细胞的处理导致无机磷酸盐和紫外线吸收材料的大量损失。扫描电子显微镜图像显示葡萄糖酸处理过的白线单胞菌细胞完全裂解。这些结果表明该肽的作用方式涉及改变膜完整性和增加渗透性，导致细胞完全裂解。物理化学表征证明了高温下生物活性的稳定性，包括高压灭菌条件和低 pH 值。用蛋白酶和一些表面活性剂处理后活性降低，用乙二胺四乙酸 (EDTA) 消除。3D 构象模型表明存在疏水区域的可能性，该区域可能直接与目标细菌的细胞膜相互作用。总之，葡糖酸抑制具有重要农业意义的植物病原体生长的能力为生物技术应用开辟了新的机会，从而减少了农药的使用。

更新日期：2021-07-27

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