Recent Advances in Plasmonic Nanostructures Applied for Label-free Single-cell Analysis,Electroanalysis

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Recent Advances in Plasmonic Nanostructures Applied for Label-free Single-cell Analysis
Electroanalysis ( IF 2.7 ) Pub Date : 2021-07-27 , DOI: 10.1002/elan.202100330
Xue-Wei Liao ₁ , Qiu-Yang Xu ₁ , Zheng Tan ₁ , Yang Liu ₁ , Chen Wang ₁

Affiliation

Cellular heterogeneity presents a major challenge in understanding the relationship between cells of particular genotype and response in disease. In order to characterize the cell-to-cell differences during the biochemical processes, single-cell analysis is necessary. Profiting from the unique localized surface plasmon resonance (LSPR) and Mie scattering, plasmonic nanostructures have revealed stable and adjustable scattering signals, avoiding photobleaching, blinking and autofluorescence phenomenon. These characterizations are propitious to the dynamic trace and biological image of single living cells. In this review, we discuss the recent advances in plasmonic nanostructures applied for label-free detection and monitoring of target cells at single-cell level by using three different techniques, surface-enhanced Raman scattering (SERS), surface-enhanced Infrared absorption spectroscopy (SEIRAS), and dark-field microscopy. Various avenues to design plasmonic probes combining spectra and imaging for single-cell analysis are demonstrated as well. We hope this review can highlight the superiority of plasmonic nanostructures in single cellular analysis, and further motivate the development of label-free cell analysis technique to elucidate cellular diversity and heterogeneity.

中文翻译：

用于无标记单细胞分析的等离子体纳米结构的最新进展

细胞异质性对理解特定基因型细胞与疾病反应之间的关系提出了重大挑战。为了表征生化过程中细胞间的差异，需要进行单细胞分析。得益于独特的局部表面等离子体共振 (LSPR) 和 Mie 散射，等离子体纳米结构显示出稳定且可调节的散射信号，避免了光漂白、闪烁和自发荧光现象。这些表征有利于单个活细胞的动态轨迹和生物学图像。在这篇综述中，我们通过使用表面增强拉曼散射 (SERS)、表面增强拉曼散射 (SERS)、表面增强拉曼散射 (SERS)、表面增强红外吸收光谱（SEIRAS）和暗场显微镜。还展示了设计用于单细胞分析的光谱和成像的等离子体探针的各种途径。我们希望这篇综述能够突出等离子体纳米结构在单细胞分析中的优越性，并进一步推动无标记细胞分析技术的发展，以阐明细胞的多样性和异质性。

更新日期：2021-07-27

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