In this study, we developed a real-time quantitative polymerase chain reaction (qPCR) assay based on a dual-labeled hydrolysis probe to simultaneously detect both duck circovirus (DuCV) 1 and DuCV-2. The reproducibility, sensitivity and specificity of the primer set and probe were evaluated using other duck pathogens. The detection limit was 20 copies per µL. The intra-assay coefficients of variation (CVs) were ≤ 0.73% and the inter-assay CVs were ≤ 1.89%. No cross-reaction occurred with other duck pathogens. In addition, the qPCR assay was successfully applied to the simultaneous detection of DuCV-1 and DuCV-2 in clinical field samples. Therefore, this assay will be useful for laboratory diagnosis and epidemiological field studies of DuCV.

在本研究中，我们开发了一种基于双标记水解探针的实时定量聚合酶链反应 (qPCR) 检测方法，可同时检测鸭圆环病毒 (DuCV) 1 和 DuCV-2。使用其他鸭病原体评估引物组和探针的再现性、灵敏度和特异性。检测限为每微升 20 个拷贝。测定内变异系数 (CV) ≤ 0.73%，测定间 CV ≤ 1.89%。与其他鸭病原体未发生交叉反应。此外，qPCR 测定已成功应用于临床现场样品中 DuCV-1 和 DuCV-2 的同时检测。因此，该测定将有助于 DuCV 的实验室诊断和流行病学现场研究。