Anaerobic ammonium oxidation (Anammox) bacteria (AnAOB) are beneficial for the treatment of NH₄⁺-N and NO₂⁻-N simultaneously in sewage waste. These bacteria form particles by secreting or capturing substances such as extracellular polymeric substances (EPS), which adsorb harmful ions with the help of charge adsorption and complexation to protect the growth of AnAOB. However, the substrate also has an electric charge, and the effect of EPS on the adsorption and mass transfer of NH₄⁺-N and NO₂⁻-N remains to be elucidated. In this study, we investigated the adsorption effect of EPS from AnAOB on different substrates by using a mathematical model and determined the possible role of EPS in bacterial growth. EPS proteins, which were extracted using the ultrasound method and detected by proteomics, were consistent with the proteins involved in the anammox reaction in cells. The detection models to calculate the adsorption capacity were established in accordance with the dialysis principle. Both the Freundlich model and Langmuir model were used to explain the adsorption of NH₄⁺-N and NO₂⁻-N by EPS proteins. Compared with the adsorption of NH₄⁺-N by EPS, that of NO₂⁻-N by EPS was well-fitted in the Langmuir model, with relatively high maximum adsorption concentrations.

厌氧氨氧化（Anammox）细菌（AnAOB）有利于同时处理污水中的NH ₄ ⁺ -N 和NO ₂ ^- -N。这些细菌通过分泌或捕获胞外聚合物（EPS）等物质形成颗粒，借助电荷吸附和络合作用吸附有害离子，保护 AnAOB 的生长。然而，底物也带有电荷，EPS对NH ₄ ⁺ -N和NO ₂ ^-的吸附和传质的影响-N 仍有待阐明。在这项研究中，我们通过使用数学模型研究了来自 AnAOB 的 EPS 对不同底物的吸附作用，并确定了 EPS 在细菌生长中的可能作用。EPS 蛋白采用超声法提取并通过蛋白质组学检测，与参与细胞内厌氧氨氧化反应的蛋白一致。根据透析原理建立了计算吸附量的检测模型。Freundlich模型和Langmuir模型均用于解释EPS蛋白对NH ₄ ⁺ -N和NO ₂ ^- -N的吸附。与EPS吸附NH ₄ ⁺ -N相比，NO ₂ ^-EPS 的 -N 非常适合 Langmuir 模型，具有相对较高的最大吸附浓度。