Abstract

In the present study, two new Co(II)-based coordination polymers with the chemical formulae of [Co₂(chtc)(H₂O)]·3H₂O (1) and [Co₂(chtc)(4,4′-bpy)_0.5] (2) have been successfully prepared via a solvothermal reaction of Co(NO₃)₂·6H₂O and cyclohexane-1e,2a,4a,5e-tetracarboxylic acid (H₄chtc) without or with the auxiliary N-donor ligand 4,4′-bipyridine (4,4′-bpy). The as-prepared two coordination polymers have been studied via the IR spectra, elemental analyses, thermogravimetric analyses, powder X-ray diffraction study, and single-crystal X-ray diffraction analyses. Furthermore, the treatment effect of compounds 1 and 2 on the CVB3 induced viral myocarditis was evaluated in vivo and the specific mechanism was explored. The Creatine Kinase detection kit was performed to detect the content of the Creatine Kinase (CK-MB) in the myocardial cells. Besides, the real time reverse transcription-polymerase chain reaction was conducted and the relative expression level of the NF-κB in myocardial cells was determined.

摘要

在本研究中，两种新的 Co(II) 基配位聚合物的化学式为 [Co ₂ (chtc)(H ₂ O)]·3H ₂ O ( 1 ) 和 [Co ₂ (chtc)(4,4 '-bpy) _0.5 ] ( 2 ) 通过 Co(NO ₃ ) ₂ ·6H ₂ O 与环己烷-1 e ,2 a ,4 a ,5 e-四羧酸 (H ₄chtc) 没有或有辅助 N 供体配体 4,4'-联吡啶 (4,4'-bpy)。通过红外光谱、元素分析、热重分析、粉末 X 射线衍射研究和单晶 X 射线衍射分析研究了所制备的两种配位聚合物。此外，在体内评价了化合物1和2对CVB3诱导的病毒性心肌炎的治疗效果并探索了具体机制。肌酸激酶检测试剂盒用于检测心肌细胞中肌酸激酶（CK-MB）的含量。此外，进行实时逆转录-聚合酶链反应，测定心肌细胞中NF-κB的相对表达水平。