ABSTRACT

Green copper nanoparticles (M-Cu NPs) were successfully synthesised by using the extract of Mentha pulegium plant, a flowering plant in the family of Lamiaceae. The synthesised M-Cu NPs were characterized by advanced technical tools. The maximum absorbance, λmax was found to be 754 nm for M-Cu NPs. The morphological analysis substantiated the spherical shape for NPs with particle size range of 21‒48 nm. The plant extract and synthesised Cu NPs exhibited potential antibacterial activity against the bacterial pathogens, Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumonia. The maximum zone of inhibition of 12 mm was recorded against B. cereus bacteria. The cell cytotoxicity of M-Cu NPs on normal human lymphocytes was assessed by methylthiazolyldiphenyl-tetrazolium bromide (MTT). M-Cu NPs demonstrated a significant antibacterial activity against S. aureus and B. cereus and almost 98% of normal human peripheral blood cells were found to survive up to 25 μg/mL of M-Cu NPs.

摘要

使用唇形科开花植物薄荷属植物的提取物成功合成了绿色铜纳米粒子（M-Cu NPs）。合成的 M-Cu NPs 以先进的技术工具为特征。M-Cu NPs 的最大吸光度 λmax 为 754 nm。形态分析证实了粒径范围为 21-48 nm 的 NPs 呈球形。植物提取物和合成的 Cu NPs 对细菌病原体、金黄色葡萄球菌、蜡状芽孢杆菌、大肠杆菌和肺炎克雷伯菌具有潜在的抗菌活性。对蜡状芽孢杆菌细菌的最大抑制区为 12 mm。通过甲基噻唑基二苯基溴化四唑 (MTT) 评估 M-Cu NPs 对正常人淋巴细胞的细胞毒性。