Damage from myocardial infarction (MI) and subsequent heart failure are serious public health concerns. Current clinical treatments and therapies to treat MI damage largely do not address the regeneration of cardiomyocytes. In a previous study, we established that it is possible to promote regeneration of cardiac muscle with vascular endothelial growth factor B gene delivery directly to the ischemic myocardium. In the current study we aim to optimize cardioporation parameters to increase expression efficiency by varying electrode configuration, applied voltage, pulse length, and plasmid vector size. By using a surface monopolar electrode, optimized pulsing conditions and reducing vector size, we were able to prevent ventricular fibrillation, increase survival, reduce tissue damage, and significantly increase gene expression levels.

心肌梗塞 (MI) 和随后的心力衰竭造成的损害是严重的公共卫生问题。目前用于治疗 MI 损伤的临床治疗和疗法在很大程度上并未解决心肌细胞的再生问题。在之前的一项研究中，我们确定可以通过将血管内皮生长因子 B 基因直接递送至缺血心肌来促进心肌的再生。在目前的研究中，我们旨在通过改变电极配置、施加电压、脉冲长度和质粒载体大小来优化心脏参数以提高表达效率。通过使用表面单极电极、优化脉冲条件和减小载体大小，我们能够预防心室颤动、提高存活率、减少组织损伤并显着提高基因表达水平。