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The effect of reversible permeabilization and post-electroporation resting on the survival of Thai basil (O. Basilicum cv. thyrsiflora) leaves during drying
Bioelectrochemistry ( IF 4.8 ) Pub Date : 2021-07-27 , DOI: 10.1016/j.bioelechem.2021.107912
Grant Thamkaew 1 , Lars Wadsö 2 , Allan G Rasmusson 3 , Federico Gómez Galindo 1
Affiliation  

Horticultural crops have a low tolerance to dehydration. In this paper, we show that the reversible electroporation (200 monopolar, rectangular pulses of 50 µs pulse duration, 760 µs between pulses and nominal field strength of 650 V/cm) of Thai basil leaves followed by 24 h resting before hot air drying at 40 °C enhanced the survivability of the tissues at certain levels of dehydration (moisture ratio = 0.2 and 0.1). However, this increased survival was rather limited. Through measurements of metabolic heat production during resting, rehydration kinetics, respiration and photosynthesis of the rehydrated leaves, we show that resting after the application of a reversible pulse-electric field (PEF) may allow a phase of hardening that has a protective effect on the cells, thus decreasing damage during the subsequent drying phase. Increased preservation of cell vitality would be associated with a more turgid and fresh-like rehydrated product, as cells would have the capacity to retain the rehydration water.



中文翻译:

可逆透化和电穿孔后对泰国罗勒 (O. Basilicum cv. thyrsiflora) 叶子在干燥过程中存活的影响

园艺作物对脱水的耐受性较低。在本文中,我们展示了泰国罗勒叶的可逆电穿孔(200 个单极、50 µs 脉冲持续时间的矩形脉冲,脉冲之间为 760 µs,标称场强为 650 V/cm),然后在热风干燥前静置 24 小时。 40 °C 提高了组织在一定程度的脱水(水分比 = 0.2 和 0.1)下的生存能力。然而,这种增加的存活率是相当有限的。通过测量再水合叶片在静息过程中产生的代谢热、再水合动力学、呼吸作用和光合作用,我们表明,在应用可逆脉冲电场 (PEF) 后静息可能会出现一个硬化阶段,从而对叶片有保护作用。细胞,从而减少后续干燥阶段的损伤。

更新日期:2021-08-04
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