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Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection
Electrophoresis ( IF 3.0 ) Pub Date : 2021-07-26 , DOI: 10.1002/elps.202100122
Saara Mikkonen 1 , Åsa Emmer 1
Affiliation  

CE-C4D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin-based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE-C4D was also, for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.

中文翻译:

使用非接触式电导检测的毛细管电泳分析细胞培养上清液中的氨基酸

介绍了用于分析氨基酸 (AA) 的CE-C 4 D 方法。将两种方法的结果与基于乙酸和环糊精的 BGE 相结合,可以使用 CE 分析 20 种蛋白质氨基酸。CE-C 4 D 还首次应用于分析哺乳动物细胞培养上清液样品中的游离氨基酸。稀释后仅作为样品制备,结合两种 CE 方法的结果,可以监测 CHO 细胞培养过程中采集的样品中 17 种 AA 的浓度变化。
更新日期:2021-07-26
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