We aimed to assess the effect of feeding Bacillus subtilis C-3102 on the growth and rumen microbiota in the preweaned calves. Twelve newborn Japanese Black calves were randomly allocated to either the control (n = 6) or the treatment (n = 6) groups in the present study. Calves in the treatment group were offered B. subtilis C-3102 supplemented milk replacer throughout the preweaning period. Rumen fermentation during the first 21 days of life seemed to be slightly suppressed by feeding B. subtilis C-3102. This fermentation shift was probably attributed to the lower abundance of the core members of rumen microbiota until 21 days of age in the calves fed B. subtilis C-3102. However, feeding B. subtilis C-3102 did not influence the abundance of the core members of rumen microbiota at 90 days of age. Distribution of Sharpea spp. and Megasphaera spp., which potentially contribute to low methane production and are regarded as beneficial rumen bacteria, was higher in the rumen of calves fed B. subtilis C-3102 at 90 days of age. These results suggest that B. subtilis C-3102 supplementation in milk replacer could potentially contribute to the improvement of feed efficiency after weaning via the establishment of beneficial rumen bacteria.

中文翻译：

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代乳粉中添加枯草芽孢杆菌 C-3102 对断奶前犊牛生长和瘤胃微生物群的影响

我们旨在评估饲喂枯草芽孢杆菌C-3102 对断奶前犊牛生长和瘤胃微生物群的影响。 在本研究中，12 只新生日本黑牛被随机分配到对照组 ( n  = 6) 或治疗组 ( n = 6)。治疗组的小牛在整个断奶前期间都被提供了B. subtilis C-3102 补充代乳品。饲喂枯草芽孢杆菌C-3102 似乎会轻微抑制出生后 21 天的瘤胃发酵。这种发酵转变可能是由于饲喂枯草芽孢杆菌的小牛在 21 日龄之前瘤胃微生物群核心成员的丰度较低C-3102。然而，饲喂枯草芽孢杆菌C-3102 并不影响 90 日龄时瘤胃微生物群核心成员的丰度。Sharpea spp的分布。和Megasphaera spp.，它们可能导致甲烷产量低，被认为是有益的瘤胃细菌，在 90 日龄饲喂枯草芽孢杆菌C-3102的小牛的瘤胃中含量较高。这些结果表明，在代乳粉中添加枯草芽孢杆菌C-3102 可能有助于通过建立有益的瘤胃细菌来提高断奶后的饲料效率。