γδ T cells constitute a major portion of lymphocytes in the blood of both ruminants and swine. Subpopulations of swine γδ T cells have been distinguished by CD2 and CD8α expression. However, it was not clear if they have distinct expression profiles of their T-cell receptor (TCR) or WC1 genes. Identifying receptor expression will contribute to understanding the functional differences between these subpopulations and their contributions to immune protection. Here, we annotated three genomic assemblies of the swine TCRγ gene locus finding four gene cassettes containing C, J and V genes, although some haplotypes carried a null TRGC gene (TRGC4). Genes in the TRGC1 cassette were homologs of bovine TRGC5 cassette while the others were not homologous to bovine genes. Here we evaluated three principal populations of γδ T cells (CD2⁺/SWC5^-, CD2^-/SWC5⁺, and CD2^-/SWC5^-). Both CD2⁻ subpopulations transcribed WC1 co-receptor genes, albeit with different patterns of gene expression but CD2⁺ cells did not. All subpopulations transcribed TCR genes from all four cassettes, although there were differences in expression levels. Finally, the CD2⁺ and CD2⁻ γδ T-cell populations differed in their representation in various organs and tissues, presumably at least partially reflective of different ligand specificities for their receptors.

γδ T 细胞构成反刍动物和猪血液中淋巴细胞的主要部分。猪 γδ T 细胞的亚群已通过 CD2 和 CD8α 表达来区分。然而，尚不清楚它们的 T 细胞受体 (TCR) 或 WC1 基因是否具有不同的表达谱。识别受体表达将有助于了解这些亚群之间的功能差异及其对免疫保护的贡献。在这里，我们注释了猪 TCRγ 基因座的三个基因组组装，发现了四个包含 C、J 和 V 基因的基因盒，尽管一些单倍型携带一个无效的 TRGC 基因 (TRGC4)。TRGC1 盒中的基因是牛 TRGC5 盒的同源物，而其他基因与牛基因不同源。在这里，我们评估了三个主要的 γδ T 细胞群（CD2⁺ /SWC5 ^-、CD2 ^- /SWC5 ⁺和 CD2 ^- /SWC5 ^- )。两个 CD2 ^-亚群都转录 WC1 共受体基因，尽管基因表达模式不同，但 CD2 ⁺细胞没有。尽管表达水平存在差异，但所有亚群都从所有四个盒中转录了 TCR 基因。最后，CD2 ⁺和 CD2 ^- γδ T 细胞群在不同器官和组织中的表现不同，可能至少部分反映了它们受体的不同配体特异性。