Biofilms can constitute permanent threats to food safety and public health. Bacteria and viruses lodged in biofilm can escape cleaning and sanitizing agents. The aim of this study was to compare Pseudomonas aeruginosa developing and mature biofilms produced on agri-food surfaces in terms of interaction with murine norovirus. Whether they were mature or still developing the biofilms apparently accumulated murine norovirus in large numbers after 24 h of contact with medium which viral titer was 2.6 × 10⁴ pfu ml⁻¹ (≈ 8 log₁₀ genome copies ml⁻¹). This appeared unrelated to surfaces’ nature and bacterial viable count but related to polysaccharide and protein contents. Virus releases may also occur mainly in connection with P. aeruginosa biofilm dispersal systems. These findings suggest that the effectiveness of surface cleaning agents and procedures for reducing the risks of biofilms-related viral contaminations need to be re-evaluated in relation with biofilm components. However, more repetitions and further in-depth specific studies are needed for confirmation of these findings and more clarifications on virus-biofilm interaction phenomenon.

生物膜可能对食品安全和公众健康构成永久性威胁。滞留在生物膜中的细菌和病毒可以逃脱清洁剂和消毒剂。本研究的目的是比较在农业食品表面产生的绿脓杆菌发育和成熟生物膜与鼠诺如病毒的相互作用。^{无论它们是成熟的还是仍在发育的生物膜在与病毒滴度为 2.6 × 10 4} pfu ml ^-1 (≈ 8 log ₁₀基因组拷贝 ml ^-1的培养基接触 24 小时后明显积累了大量鼠诺如病毒）。这似乎与表面的性质和细菌存活计数无关，但与多糖和蛋白质含量有关。病毒释放也可能主要与铜绿假单胞菌生物膜扩散系统有关。这些发现表明，需要针对生物膜成分重新评估表面清洁剂和降低生物膜相关病毒污染风险的程序的有效性。然而，需要更多的重复和进一步深入的具体研究来证实这些发现和对病毒-生物膜相互作用现象的更多澄清。