Enteric viruses have been described as important contaminants in fresh and ready-to-eat foods such as sandwiches, deli meat and dairy products. This is a cross-sectional randomized survey to estimate the prevalence of norovirus and human adenovirus (HAdV) from 100 Brazilian artisanal raw milk cheese samples (Minas and Coalho) obtained from different agroindustries in four producing regions in the states of Minas Gerais and one in Piauí, respectively. From October 2017 to April 2018, norovirus genogroups I and II and HAdV were investigated in these cheese samples by RT-qPCR and qPCR, respectively. Viruses were detected in 43 samples, being 26 norovirus GI strains, 14 HAdV, and 3 both viruses. Norovirus GII strains were not detected. Viral concentrations ranged from 6.17 × 10⁴ to 1.44 × 10⁷ genome copies/L^–1 and murine norovirus 1 used as internal process control showed 100% success rate of recovery with efficiency of 10%. There was a trend towards a higher positivity rate for both viruses in the rainy season, and HAdV were more commonly found among samples with higher fecal coliform counts. This study is a first step in assessing the risk that this contamination may pose to the consumer of raw products as well as emphasizing the need for good manufacturing practices, quality control systems in the dairy industry and markets. As a randomized survey, we established baseline figures for viruses’ prevalence in five types of ready-to-eat raw milk artisanal Brazilian cheese, to allow any monitoring trends, setting control targets and future local risk analyses studies.

肠道病毒被描述为三明治、熟肉和奶制品等新鲜和即食食品中的重要污染物。这是一项横断面随机调查，旨在估计来自米纳斯吉拉斯州四个生产区和一个在皮奥伊，分别。从 2017 年 10 月到 2018 年 4 月，分别通过 RT-qPCR 和 qPCR 对这些奶酪样品中的诺如病毒基因组 I 和 II 和 HAdV 进行了研究。在 43 个样本中检测到病毒，包括 26 种诺如病毒 GI 株、14 种 HAdV 和 3 种两种病毒。未检测到诺如病毒 GII 株。病毒浓度范围为 6.17 × 10 ⁴至 1.44 × 10 ⁷基因组拷贝数/L ^–1和鼠诺如病毒 1 用作内部过程控制显示 100% 的成功回收率和 10% 的效率。在雨季，两种病毒的阳性率均呈较高趋势，而在粪便大肠菌群计数较高的样本中，HAdV 更常见。这项研究是评估这种污染可能对原料消费者造成的风险的第一步，并强调了乳制品行业和市场对良好生产规范、质量控制系统的需求。作为一项随机调查，我们建立了五种即食生乳手工巴西奶酪中病毒流行的基线数据，以允许任何监测趋势、设定控制目标和未来的当地风险分析研究。