In this study, we have identified a novel peptide NV14 with antioxidative functions from serine O-acetyltransferase (SAT) of Artrospira platensis (Ap). The full sequence of ApSAT and its derived NV14 peptide “NVRIGAGSVVLRDV” (141–154) was characterized using bioinformatics tools. To address the transcriptional activity of ApSAT in response to induce generic oxidative stress, the spirulina culture was exposed to H₂O₂ (10 mM). The ApSAT expression was studied using RT-PCR across various time points and it was found that the expression of the ApSAT was significantly upregulated on Day 15. The in vitro cytotoxicity assay against NV14 was performed in human dermal fibroblast cells and human blood leukocytes. Results showed that NV14 treatment was non-cytotoxic to the cells. Besides, in vivo treatment of NV14 in zebrafish larvae did not exhibit the signs of developmental toxicity. Further, the in vitro antioxidant assays enhanced the activity of the antioxidant enzymes, such as SOD and CAT, due to NV14 treatment; and also significantly reduced the MDA levels, while increasing the superoxide radical and H₂O₂ scavenging activity. The expression of antioxidant enzyme genes glutathione peroxidase, γ-glutamyl cysteine synthase, and glutathione S-transferase were found to be upregulated in the NV14 peptide pretreated zebrafish larvae when induced with generic oxidative stress, H₂O₂. Overall, the study showed that NV14 peptide possessed potent antioxidant properties, which were demonstrated over both in vitro and in vivo assays. NV14 enhanced the expression of antioxidant enzyme genes at the molecular level, thereby modulating and reversing the cellular antioxidant balance disrupted due to the H₂O₂-induced oxidative stress.

中文翻译：

---

来自蓝藻丝氨酸 O-乙酰转移酶的 NV14 影响体外细胞的抗氧化酶、抗 H2O2 的基因表达和体内斑马鱼幼虫模型的其他反应

在这项研究中，我们从青蒿( Ap ) 的丝氨酸O-乙酰转移酶(SAT) 中鉴定了一种具有抗氧化功能的新型肽NV14。使用生物信息学工具表征Ap SAT 及其衍生的 NV14 肽“NVRIGAGSVVLRDV”（141-154）的完整序列。为了解决Ap SAT 响应诱导一般氧化应激的转录活性，将螺旋藻培养物暴露于 H ₂ O ₂ (10 mM)。使用 RT-PCR 研究不同时间点的Ap SAT 表达，发现Ap的表达SAT 在第 15 天显着上调。针对 NV14 的体外细胞毒性试验在人真皮成纤维细胞和人血白细胞中进行。结果表明，NV14 处理对细胞没有细胞毒性。此外，在斑马鱼幼虫体内处理 NV14 并没有表现出发育毒性的迹象。此外，由于 NV14 处理，体外抗氧化试验增强了 SOD 和 CAT 等抗氧化酶的活性；并且还显着降低了MDA水平，同时增加了超氧自由基和H ₂ O ₂清除活性。抗氧化酶基因谷胱甘肽过氧化物酶、γ-谷氨酰半胱氨酸合酶和谷胱甘肽S的表达_{当用一般氧化应激 H 2} O ₂诱导时，发现经 NV14 肽预处理的斑马鱼幼虫中的β-转移酶上调。总体而言，该研究表明 NV14 肽具有有效的抗氧化特性，这在体外和体内试验中都得到了证明。NV14在分子水平上增强抗氧化酶基因的表达，从而调节和逆转由于H ₂ O ₂诱导的氧化应激而破坏的细胞抗氧化平衡。