Background

Cervical cancer is a major health problem caused by human papillomavirus (HPV) infection which affects more than 500,000 women every year worldwide. Majority of the cases occur in less developed countries since there are lack of availability of effective screening methods for diagnosis. Therefore, a fast, accurate and early detection platform is developed for HPV detection. The current study describes a sensitive, specific, easy and a cost-effective label free DNA based electrochemical biosensor for HPV-16 detection.

Methodology

Detection of HPV-16 is performed by an electrochemical platform coated with chitosan capped gold nanoparticles (ccAuNPs). These nanoparticles were characterized using Ultraviolet–visible (UV–vis) analysis, Fourier Transfer Infrared Ray analysis (FT-IR) and Transmission Electron Microscope (TEM). The probe DNA (PDNA) was then immobilized onto the nanoparticle modified electrode and hybridized with target DNA (TDNA). The electrochemical analysis was done at each step of modification using cyclic voltammetry (CV) and square wave voltammetry (SWV).

Results

The biosensor responds to TDNA concentration range from 1 pM to 1 μM and the limit of detection is 1 pM with the sensitivity of 0.39 mA pM⁻¹. This proposed biosensor achieved good performance of the PDNA/ccAuNPs modified ITO electrode.

Conclusions

The results illustrate that the developed nanotechnology-based electrochemical DNA biosensor provides very low detection limit in picomolar level. It also exhibited excellent selectivity and stability, which is important for potential applications in the onsite analysis of HPV-16 and in medical diagnosis.

中文翻译：

---

通过超痕量 DNA 杂交分析检测宫颈癌的无标记纳米传感平台

背景

宫颈癌是由人乳头瘤病毒 (HPV) 感染引起的主要健康问题，每年影响全球超过 500,000 名妇女。大多数病例发生在欠发达国家，因为缺乏有效的诊断筛查方法。因此，为HPV检测开发了一个快速、准确和早期的检测平台。目前的研究描述了一种用于 HPV-16 检测的灵敏、特异、简单且经济高效的基于无标记 DNA 的电化学生物传感器。

方法

HPV-16 的检测是通过涂有壳聚糖封端的金纳米粒子 (ccAuNPs) 的电化学平台进行的。这些纳米粒子使用紫外-可见光 (UV-vis) 分析、傅里叶传输红外分析 (FT-IR) 和透射电子显微镜 (TEM) 进行表征。然后将探针 DNA (PDNA) 固定在纳米粒子修饰电极上并与目标 DNA (TDNA) 杂交。在使用循环伏安法 (CV) 和方波伏安法 (SWV) 的每个修改步骤中进行电化学分析。

结果

生物传感器响应从 1 pM 到 1 μM 的 TDNA 浓度范围，检测限为 1 pM，灵敏度为 0.39 mA pM ^-1。这种提出的生物传感器实现了 PDNA/ccAuNPs 修饰的 ITO 电极的良好性能。

结论

结果表明，开发的基于纳米技术的电化学 DNA 生物传感器提供了非常低的皮摩尔级检测限。它还表现出出色的选择性和稳定性，这对于 HPV-16 现场分析和医学诊断的潜在应用非常重要。