N1-methyladenosine (m1A), is a unique methyl group that confers post-transcriptional modification of gene expression, and plays important roles in various human diseases. However, the abundance of this modification and its effects on long non-coding RNAs (lncRNAs) in human colorectal cancer (CRC) remain unclear. In this study, methylated RNA immunoprecipitation sequencing was performed in three pairs of human CRC and nontumorous tissues to identify m1A peaks and its correlation with differential alterations of lncRNA expression in CRC. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment pathway analyses were applied to predict the potential roles of m1A on gene expression. We found that CRC and adjacent tissues had a noticeable difference in m1A distribution. Notably, HGGAGRA and WGGANGA were recognized as the most significantly enriched motifs, respectively. Co-analysis of methylation and RNA sequencing demonstrated downregulated lncRNAs along with m1A modification in CRC. GO and KEGG pathway analyses revealed that the unique distribution of m1A sites in lncRNAs had a significant correlation with CRC signaling pathways. In conclusion, our results delineated the distribution pattern of m1A methylation on lncRNAs, and provided potential roles of this modification in different pathways and tumor progression of CRC.

中文翻译：

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结直肠癌中长链非编码 RNA 的 N1-甲基腺苷谱分析

N1-甲基腺苷 (m1A) 是一种独特的甲基，可对基因表达进行转录后修饰，在多种人类疾病中发挥重要作用。然而，这种修饰的丰度及其对人类结直肠癌 (CRC) 中长链非编码 RNA (lncRNA) 的影响仍不清楚。在这项研究中，在三对人类 CRC 和非肿瘤组织中进行甲基化 RNA 免疫沉淀测序，以确定 m1A 峰及其与 CRC 中 lncRNA 表达差异变化的相关性。应用基因本体论 (GO) 和京都基因和基因组百科全书 (KEGG) 富集途径分析来预测 m1A 对基因表达的潜在作用。我们发现 CRC 和邻近组织在 m1A 分布上有显着差异。尤其，HGGAGRA 和 WGGANGA 分别被认为是最显着丰富的基序。甲基化和 RNA 测序的共同分析表明，在 CRC 中 lncRNA 下调以及 m1A 修饰。GO和KEGG通路分析表明，lncRNA中m1A位点的独特分布与CRC信号通路存在显着相关性。总之，我们的结果描绘了 lncRNA 上 m1A 甲基化的分布模式，并提供了这种修饰在 CRC 不同途径和肿瘤进展中的潜在作用。GO和KEGG通路分析表明，lncRNA中m1A位点的独特分布与CRC信号通路存在显着相关性。总之，我们的结果描绘了 lncRNA 上 m1A 甲基化的分布模式，并提供了这种修饰在 CRC 不同途径和肿瘤进展中的潜在作用。GO和KEGG通路分析表明，lncRNA中m1A位点的独特分布与CRC信号通路存在显着相关性。总之，我们的结果描绘了 lncRNA 上 m1A 甲基化的分布模式，并提供了这种修饰在 CRC 不同途径和肿瘤进展中的潜在作用。