Alpha-chloralose (AC) is used as a rodenticide as well as an anesthetic agent in laboratory animals. It was previously also used as an avicide. Detection of AC in blood samples or in body tissues collected postmortem is key for the diagnosis of clinical cases and a requirement for surveillance of secondary toxicosis, including potential cases in wild animals. Reports on poisoning of humans and non-laboratory animals confirmed by the detection of AC or its metabolites are available, however poisoning of domestic animals are rarely available. Furthermore, reports on clinical cases in domestic animals rarely report quantifications of AC in blood or body tissues. The present study describes the validation of a quantitative Ultrahigh Performance Liquid Chromatography tandem Mass Spectrometry (UHPLC-MS-MS) method that can be used in cases of suspected AC poisoning in cats. The validation study showed the method to be fit for purpose. In serum, the limit of quantification was 100 ng/mL and the limit of detection was 30 ng/mL. The new analytical method was applied on blood samples collected from 20 individual cats with a preliminary clinical diagnosis of acute AC poisoning. AC was confirmed in all 20 feline blood samples, and the concentration range of AC was 538–17,500 ng/mL. The quantitative method developed in this study was found to be a fast and selective method for confirmation of AC poisoning using blood samples from cats.

中文翻译：

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用于测定猫血中 α-氯醛糖的定量 UHPLC-MS-MS 方法的开发和验证以及在从有 α-氯醛糖中毒症状的猫采集的血液样本中的应用

α-氯醛糖 (AC) 在实验动物中用作灭鼠剂和麻醉剂。它以前也被用作杀鸟剂。在血液样本或死后收集的身体组织中检测 AC 是诊断临床病例的关键，也是监测继发性中毒（包括野生动物的潜在病例）的必要条件。有经检测到 AC 或其代谢物证实的人类和非实验室动物中毒的报告，但很少有家畜中毒的报道。此外，关于家畜临床病例的报告很少报告血液或身体组织中 AC 的量化。本研究描述了一种定量超高效液相色谱串联质谱 (UHPLC-MS-MS) 方法的验证，该方法可用于猫疑似 AC 中毒的情况。验证研究表明该方法适合目的。在血清中，定量限为 100 ng/mL，检测限为 30 ng/mL。将新的分析方法应用于从 20 只初步临床诊断为急性 AC 中毒的猫身上采集的血液样本。在所有 20 份猫血样中均确认了 AC，AC 的浓度范围为 538–17,500 ng/mL。发现本研究中开发的定量方法是一种使用猫血样确认 AC 中毒的快速且选择性的方法。在血清中，定量限为 100 ng/mL，检测限为 30 ng/mL。将新的分析方法应用于从 20 只初步临床诊断为急性 AC 中毒的猫身上采集的血液样本。在所有 20 份猫血样中均确认了 AC，AC 的浓度范围为 538–17,500 ng/mL。发现本研究中开发的定量方法是一种使用猫血样确认 AC 中毒的快速且选择性的方法。在血清中，定量限为 100 ng/mL，检测限为 30 ng/mL。将新的分析方法应用于从 20 只初步临床诊断为急性 AC 中毒的猫身上采集的血液样本。在所有 20 份猫血样中均确认了 AC，AC 的浓度范围为 538–17,500 ng/mL。发现本研究中开发的定量方法是一种使用猫血样确认 AC 中毒的快速且选择性的方法。