Abstract

The determination of anthocyanins and anthocyanidins in wild grapes (Vitis sylvestris Gmelin) from Tunceli-Turkey are reported together with the influence of the extraction conditions on the results. Representative sets of anthocyanins (delphinidin-3-o-glucoside, cyanidin-3-o-glucoside, pelargonidin-3-o-glucoside, malvidin-3-o-glucoside) and anthocyanidins (delphinidin, cyanidin, pelargonidin, malvidin) were simultaneously determined by high performance liquid chromatography with diode array detection (HPLC-DAD). The extraction conditions including the solvent type, extraction time and liquid:solid ratio (v:w) were optimized. The anthocyanin and anthocyanidin contents changed significantly depending on the extraction conditions. Malvidin-3-o-glucoside was identified to be the primary compound.

摘要

报道了土耳其Tunceli-Turkey野生葡萄（Vitis sylvestris Gmelin）中花青素和花青素的含量测定以及提取条件对结果的影响。代表套花青素（花翠素-3- ø葡糖苷，花青素-3- ø葡糖苷，花葵素-3- ø葡糖苷，二甲花翠素-3- ö-葡萄糖苷）和花色素（飞燕草素、花青素、天竺葵素、锦葵素）同时通过高效液相色谱与二极管阵列检测器 (HPLC-DAD) 测定。优化了提取条件，包括溶剂类型、提取时间和液固比 (v:w)。花青素和花色素的含量因提取条件而异。Malvidin-3- o -glucoside被鉴定为主要化合物。