The endoplasmic reticulum transmembrane protein vesicle-associated membrane protein-associated protein (VAP) plays a central role in the formation and function of membrane contact sites (MCS) through its interactions with proteins. The major sperm protein (MSP) domain of VAP binds to a variety of sequences which are referred to as FFAT-like motifs. In this study, we investigated the interactions of eight peptides containing FFAT-like motifs with the VAP-A MSP domain (VAP-A_MSP) by solution NMR. Six of eight peptides are specifically bound to VAP-A. Furthermore, we found that the RNA-dependent RNA polymerase of severe acute respiratory syndrome coronavirus 2 has an FFAT-like motif which specifically binds to VAP-A_MSP as well as other FFAT-like motifs. Our results will contribute to the discovery of new VAP interactors.

中文翻译：

---

核磁共振揭示了 FFAT 样基序与 VAP-A 特异性结合的序列要求

内质网跨膜蛋白囊泡相关膜蛋白相关蛋白 (VAP) 通过其与蛋白质的相互作用，在膜接触位点 (MCS) 的形成和功能中起核心作用。VAP 的主要精子蛋白 (MSP) 结构域与多种序列结合，这些序列被称为 FFAT 样基序。在这项研究中，我们通过溶液核磁共振研究了含有 FFAT 样基序的八种肽与 VAP-A MSP 结构域 (VAP-A _MSP )的相互作用。八种肽中有六种与 VAP-A 特异性结合。此外，我们发现严重急性呼吸综合征冠状病毒 2 的 RNA 依赖性 RNA 聚合酶具有与 VAP-A _MSP特异性结合的 FFAT 样基序。以及其他类似 FFAT 的图案。我们的结果将有助于发现新的 VAP 相互作用物。