Abstract

Salt stress is an important factor restricting crop growth. Thus, genes related to plant responses to high salinity at the sprout stage of common bean (Phaseolus vulgaris) should be identified and characterized. In this study, on the basis of the common bean germination index, 0.4% NaCl and day 5 of the incubation period were selected as the optimal salt concentration and sampling time, respectively. Salt-tolerant and salt-sensitive plant materials suitable for the local region were selected as representative common bean materials with contrasting salt-related phenotypes. A total of 441 differentially expressed genes (DEGs) were identified by analyzing the generated RNA-seq data. The enriched Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways indicated the DEGs were associated with oxidoreductase activities and phenylpropanoid biosynthesis. Furthermore, the superoxide dismutase, peroxidase, and catalase activities and the lignin content, which are related to oxidoreductase activities and phenylpropanoid biosynthesis, were revealed to be significantly associated with common bean salt tolerance. The six DEGs with salt tolerance-related Arabidopsis thaliana homologs may be important candidate genes mediating the salt stress responses of common bean during the sprout stage.

Supplemental data for this article is available online at https://doi.org/10.1080/13102818.2021.1954091 .

摘要

盐胁迫是限制作物生长的重要因素。因此，在普通豆（菜豆）发芽阶段与植物对高盐度的反应相关的基因) 应该被识别和表征。本研究以普通豆类发芽指数为基础，分别选择0.4% NaCl和孵化期第5天作为最佳盐浓度和采样时间。选择适合当地的耐盐和盐敏感植物材料作为具有对比盐相关表型的代表性普通豆材料。通过分析生成的 RNA-seq 数据，共鉴定了 441 个差异表达基因 (DEG)。丰富的基因本体术语和京都基因和基因组途径百科全书表明 DEG 与氧化还原酶活性和苯丙烷生物合成有关。此外，超氧化物歧化酶、过氧化物酶和过氧化氢酶活性和木质素含量，与氧化还原酶活性和苯丙烷生物合成有关，被揭示与普通豆耐盐性显着相关。与耐盐性相关的六个DEG拟南芥同源物可能是重要的候选基因，介导普通豆芽阶段的盐胁迫反应。

本文的补充数据可在 https://doi.org/10.1080/13102818.2021.1954091 在线获得。