Epilepsy affects around 50 million people worldwide, and an important number of patients (30%) fail to respond to any available antiepileptic drug. Previous studies have shown that luteolin presents a promising potential as an anticonvulsant. On the other hand, different studies showed that luteolin does not promote anticonvulsant effects. Therefore, there is a lack of consensus about the use of luteolin for seizure control. Luteolin low bioavailability could be a limiting factor to obtain better results. Attractively, micronization technology has been applied to improve flavonoids bioavailability. Thus, the present study aimed to investigate the effects of luteolin on its raw form and micronized luteolin in a PTZ-induced seizure model in adult zebrafish (Danio rerio). Our results demonstrate that luteolin and micronized luteolin did not block PTZ-induced seizures in adult zebrafish. Also, luteolin and micronized luteolin did not provoke behavioral changes. Finally, our results show that 24 h after seizure occurrence, no changes were detected for p70S6Kb, interleukin 1β, and caspase-3 transcript levels. Altogether, we failed to observe an anticonvulsant potential of luteolin in adult zebrafish, even in its micronized form. However, we recommend new studies to investigate luteolin benefits in epilepsy.

癫痫影响全球约 5000 万人，并且大量患者 (30%) 对任何可用的抗癫痫药物均无反应。先前的研究表明，木犀草素作为抗惊厥药具有广阔的潜力。另一方面，不同的研究表明，木犀草素不会促进抗惊厥作用。因此，关于使用木犀草素控制癫痫发作缺乏共识。木犀草素低生物利用度可能是获得更好结果的限制因素。有吸引力的是，微粉化技术已被应用于提高类黄酮的生物利用度。因此，本研究旨在研究木犀草素对其原始形式和微粉化木犀草素在成年斑马鱼 PTZ 诱发癫痫模型中的影响。）。我们的研究结果表明，木犀草素和微粉化木犀草素不会阻止成年斑马鱼 PTZ 引起的癫痫发作。此外，木犀草素和微粉化木犀草素不会引起行为变化。最后，我们的结果表明，癫痫发作 24 小时后，p70S6Kb、白细胞介素 1β和caspase-3转录水平未检测到变化。总之，我们未能在成年斑马鱼中观察到木犀草素的抗惊厥潜力，即使是在其微粉化形式中也是如此。然而，我们建议进行新的研究来调查木犀草素对癫痫的益处。