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Molecular record for the first authentication of Isaria cicadae from Vietnam
Open Life Sciences ( IF 1.7 ) Pub Date : 2021-07-15 , DOI: 10.1515/biol-2021-0074 Thuan Duc Lao 1 , Hanh Van Trinh 2 , Loi Vuong 2, 3 , Luyen Tien Vu 1 , Thuy Ai Huyen Le 1 , Hiep Minh Dinh 4 , Nguyen Binh Truong 5
Open Life Sciences ( IF 1.7 ) Pub Date : 2021-07-15 , DOI: 10.1515/biol-2021-0074 Thuan Duc Lao 1 , Hanh Van Trinh 2 , Loi Vuong 2, 3 , Luyen Tien Vu 1 , Thuy Ai Huyen Le 1 , Hiep Minh Dinh 4 , Nguyen Binh Truong 5
Affiliation
The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae , belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including ITS , nrLSU , nrSSU , Rpb1 , and Tef1 was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify ITS , nrLSU , nrSSU , Rpb1 , and Tef1 gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus Verticillium . The phylogenetic analysis results indicated that T011 was accepted at subclade Cordyceps and significantly formed the monophyletic group with referent Cordyceps cicadae (Telemorph of Isaria cicadae ) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the Isaria cicadae . In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as Isaria cicadae .
中文翻译:
首次鉴定越南蝉的分子记录
越南多乐省咖啡园采集的寄生于蝉若虫的昆虫病原真菌T011初步形态鉴定为蝉蝉 ,属于Hypocreales目和Clavicipitaceae科。为确保 T011 的真实性,对串联的多个基因组进行系统发育分析,包括它的 ,nrLSU ,nrSSU ,rpb1 , 和铁氟龙 用于支持识别。从干燥样品 T011 中分离基因组 DNA。应用 PCR 测定测序来扩增它的 ,nrLSU ,nrSSU ,rpb1 , 和铁氟龙 基因。对于系统发育分析,两个目标基因的级联数据是使用 MEGAX 构建的,基于邻接、最大似然、最大简约法,具有 1,000 次重复引导。结果,包含62个序列的级联数据属于Hypocreales目,Clavicipitaceae科,2个外群序列属于Hypocreales,属黄萎病菌 . 系统发育分析结果表明T011在亚进化枝上被接受虫草 并与参照物显着形成单系群虫草蝉 (变形金刚蝉蝉 ) 具有高引导值。系统发育分析的结果得到了我们描述为蝉蝉 . 总之,基于级联数据集的系统发育分析已成功应用于加强 T011 的识别蝉蝉 .
更新日期:2021-07-15
中文翻译:
首次鉴定越南蝉的分子记录
越南多乐省咖啡园采集的寄生于蝉若虫的昆虫病原真菌T011初步形态鉴定为