This study reports the isolation of a potential probiotic lactic acid bacteria (LAB) from fermented Solanum macrocarpon food and evaluates shrimp bacterial pathogens' inhibition. The isolate was determined by identifying the inhibition zone against Vibrio parahaemolyticus with an antagonistic activity of 360 AU/mL. Molecular identification based on 16S rRNA indicated that the isolate belongs to the Pediococcus genus, named as Pediococcus pentosaceus HN10. The isolate exhibited effective inhibitory ability against various pathogenic including antibiotic-resistant Vibrio spp., Escherichia coli ATCC 85922 and Staphylococcus aureus ATCC 25023. P. pentosaceus HN10 showed potential probiotic properties such as strong salt tolerance, low pH and bile salts resistance, autoaggregation and coaggregation activities. P. pentosaceus HN10 was negative for β-hemolytic and amino acid decarboxylase. Antibiotic resistance assay showed that this strain was sensitive to ampicillin and chloramphenicol. P. pentosaceus HN10 expressed strongly antioxidant activity. In vivo challenge to V. parahaemolyticus in shrimp model indicated P. pentosaceus HN10 enhanced survival rate, weight gain as well as reduced the number of Vibrio in shrimp intestine tract. Overall, this study suggests that P. pentosaceus HN10 could be applied as a supplementation feed for shrimp to minimize pathogenic bacteria's negative effect.

本研究报告了从发酵的大果茄食物中分离出一种潜在的益生菌乳酸菌 (LAB)，并评估了对虾细菌病原体的抑制作用。通过鉴定具有 360 AU/mL 拮抗活性的副溶血性弧菌的抑制区来确定分离物。基于16S rRNA的分子鉴定表明该分离物属于片球菌属，命名为戊糖片球菌HN10。该分离株对多种病原体表现出有效的抑制能力，包括耐抗生素弧菌属、大肠杆菌ATCC 85922和金黄色葡萄球菌ATCC 25023。P. pentosaceus HN10显示出潜在的益生菌特性，如强耐盐性、低 pH 值和胆盐耐受性、自动聚集和共聚集活性。P. pentosaceus HN10 β-溶血酶和氨基酸脱羧酶呈阴性。抗生素抗性试验表明该菌株对氨苄青霉素和氯霉素敏感。P. pentosaceus HN10表现出强烈的抗氧化活性。在虾模型中对副溶血性弧菌的体内攻击表明戊糖酵母HN10提高了虾的存活率、体重增加以及减少了虾肠道中弧菌的数量。总的来说，这项研究表明P. pentosaceus HN10 可用作虾的补充饲料，以尽量减少病原菌的负面影响。