Fusel oil was used as a source of isoamyl alcohol in the synthesis of biolubricant by esterification with oleic acid catalyzed by different sources of lipase immobilized on poly(styrene/divinylbenzene) copolymer resin. In preliminary tests, Rhizopus oryzae lipase showed satisfactory performance, providing high molar conversion of the starting materials (>90% in 24 h of reaction), and was selected for subsequent experiments. The optimal conditions for isoamyl oleate production by immobilized R. oryzae lipase were 1:1.5 ratio of fusel oil to oleic acid and reaction temperature of 45°C. Continuous runs were carried out in a two-stage packed-bed reactor coupled to a water extraction column filled with molecular sieves. Each reactor stage was loaded with 2.6 g of immobilized lipase and then fed with fusel oil and oleic acid at a 1:1.5 molar ratio in the presence of isooctane. The performance of the proposed system was tested at a space time of 10.2 h, resulting in a mean ester productivity of 292.20 ± 18.46 µmol g⁻¹ min⁻¹ and biocatalyst half-life of 179.6 h. The properties of purified samples were in accordance with standard requirements for biolubricants.

在固定在聚（苯乙烯/二乙烯基苯）共聚物树脂上的不同来源的脂肪酶催化下，通过与油酸的酯化反应，杂醇油被用作异戊醇的来源。在初步测试中，米根霉脂肪酶表现出令人满意的性能，提供了高的起始原料摩尔转化率（在反应 24 小时内 >90%），并被选择用于后续实验。固定化稻瘟病菌生产油酸异戊酯的最佳条件脂肪酶的杂醇油与油酸的比例为 1:1.5，反应温度为 45°C。在与装有分子筛的水萃取塔相连的两级填充床反应器中进行连续运行。每个反应器阶段装载 2.6 g 固定化脂肪酶，然后在异辛烷存在下以 1:1.5 的摩尔比加入杂醇油和油酸。所提出系统的性能在10.2 小时的空间时间进行测试，导致平均酯生产率为292.20 ± 18.46 µmol g ^-1 min ^-1和179.6 小时的生物催化剂半衰期。纯化样品的特性符合生物润滑剂的标准要求。