Intrinsic neuronal activity is a hallmark of the developing brain. In rodents, a handful of such activities were described in different cortical areas but the unifying macroscopic perspective is still lacking. Here we combined large-scale in vivo Ca²⁺ imaging of the dorsal cortex in non-anesthetized neonatal mice with mathematical analyses to reveal unique behavioral state-specific maps of intrinsic activity. These maps were remarkably stable over time within and across experiments and used patches of correlated activity with little hemispheric symmetry as well as stationary and propagating waves as building blocks. Importantly, the maps recorded during motion and rest were almost inverse, with frontoparietal areas active during motion and posterior-lateral areas active at rest. The retrosplenial cortex engaged in both resting- and motion-related activities via functional long-range connections with respective cortical areas. The data obtained bind different region-specific activity patterns described so far into a single consistent picture and set the stage for future inactivation studies, probing the exact function of this complex activity pattern for cortical wiring in neonates.

内在神经元活动是大脑发育的标志。在啮齿动物中，在不同的皮层区域描述了少数此类活动，但仍缺乏统一的宏观视角。在这里，我们结合了大规模的体内Ca ²⁺通过数学分析对非麻醉新生小鼠的背侧皮层进行成像，以揭示内在活动的独特行为状态特异性图。随着时间的推移，这些地图在实验内部和实验之间非常稳定，并且使用了具有很少半球对称性的相关活动斑块以及静止和传播波作为构建块。重要的是，在运动和休息期间记录的地图几乎是相反的，在运动期间额顶区域活跃，而后外侧区域在休息时活跃。压后皮层通过与相应皮层区域的功能性远程连接参与静息和运动相关活动。获得的数据将迄今为止描述的不同区域特异性活动模式结合成一个一致的图片，并为未来的灭活研究奠定了基础，