Gestational diabetes mellitus (GDM) is the serious complication of pregnancy induced via dysfunction in glucose metabolism during the pregnancy. Crocetin already proved antidiabetic effect in streptozotocin (STZ)-induced diabetes mellitus in rats. In this protocol, we have investigated the potential effect of crocetin against STZ-induced GDM in rats. Wistar rats were used for the current protocol; STZ was used for the induction for DM and finally caused the GDM. Body weight and serum advanced glycation end products level were estimated at regular time intervals. We also estimated the fetus weight and placental weight. Biochemical, antioxidant, pro-inflammatory cytokines, inflammatory mediators, and apoptosis parameters were estimated. mRNA expression of NOX2, RAGE, MCP-1, VCAM-1, EGFR, and p65 were also estimated. Crocetin treatment significantly (p < .001) reduced the fetus weight and increased the placental weight and index. Crocetin significantly (p < .001) reduced the blood glucose level and increased the body weight. Crocetin significantly (p < .001) boosted the level of antioxidant enzymes and includes superoxide dismutase, glutathione peroxidase, glutathione, and catalase. Crocetin significantly (p < .001) altered the level of lipid parameters and pro-inflammatory cytokines. Crocetin significantly (p < .001) reduced the level of intercellular adhesion molecule 1, cyclooxygenase-2, and nuclear factor kappa B and increased the level of visfatin against GDM rats. Crocetin significantly (p < .001) altered the level of mRNA expression. Based on the result, we can say that crocetin is a protective drug against the GDM in pregnant rats via antioxidant, inflammatory, and apoptosis parameters.

中文翻译：

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藏红花素通过抑制炎症反应抑制链脲佐菌素诱导的糖尿病大鼠妊娠糖尿病

妊娠期糖尿病（GDM）是妊娠期间因糖代谢功能障碍而引起的严重妊娠并发症。藏红花素已证明对链脲佐菌素 (STZ) 诱导的大鼠糖尿病具有抗糖尿病作用。在该协议中，我们研究了藏红花素对大鼠 STZ 诱导的 GDM 的潜在影响。Wistar 大鼠用于当前方案；STZ用于诱导DM并最终引起GDM。定期评估体重和血清晚期糖基化终产物水平。我们还估计了胎儿重量和胎盘重量。估计了生化、抗氧化剂、促炎细胞因子、炎症介质和细胞凋亡参数。还估计了 NOX2、RAGE、MCP-1、VCAM-1、EGFR 和 p65 的 mRNA 表达。藏红花素处理显着（p  < .001) 降低了胎儿重量并增加了胎盘重量和指数。藏红花素显着 ( p  < .001) 降低血糖水平并增加体重。藏红花素显着 ( p  < .001) 提高了抗氧化酶的水平，包括超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽和过氧化氢酶。藏红花素显着 ( p  < .001) 改变了脂质参数和促炎细胞因子的水平。Crocetin 显着 ( p  < .001) 降低细胞间粘附分子 1、环氧合酶 2 和核因子 kappa B 的水平，并增加针对 GDM 大鼠的内脂素水平。藏红花素显着 ( p < .001) 改变了 mRNA 表达水平。基于该结果，我们可以说藏红花素是一种通过抗氧化、炎症和细胞凋亡参数对抗妊娠大鼠 GDM 的保护性药物。