Collagen hydrolysate, an extensively used protein obtained from different sources, has various beneficial effects on human health and diseases. The benefits of collagen hydrolysate are well known and presently varied sources for the preparation of hydrolysate are being investigated. Food as a therapy to combat inflammation is presently a much-focused field of research. The present study aims at screening the anti-inflammatory property of collagen hydrolysate from the skin of Cypselurus melanurus, Catla catla, Indian mackerel, Clarias batrachus (Cb), and Pangasius pangasius (Pp) in activated RAW 264.7 macrophage cells. The fractions, Cb (C2) and Pp (P2) with anti-inflammatory property obtained after two-step chromatographic purification contained peptides in the range of 1–3 kDa molecular weight. The active fractions C2 and P2 showed a reduction in gene expression of TNF-α to 1.6- and 1-fold difference, whereas IL6 expression to 30- and 40-fold difference, respectively, in comparison to LPS treatment. The suppression of inflammatory proteins (TNF-α, IL6, NFκB, and p-IκB) by fractions C2 and P2 confirmed the anti-inflammatory activity.

中文翻译：

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鱼胶原水解物在减轻炎症中的作用——一项体外研究

胶原蛋白水解物是一种从不同来源获得的广泛使用的蛋白质，对人类健康和疾病具有多种有益作用。胶原蛋白水解物的好处是众所周知的，目前正在研究用于制备水解物的各种来源。食物作为对抗炎症的疗法是目前备受关注的研究领域。本研究的目的是从皮肤筛选胶原蛋白水解物的抗炎特性Cypselurus橙线，喀拉鲃，印度鲭鱼，胡子鲶蟾（CB），和鲶鱼鲶鱼(Pp) 在激活的 RAW 264.7 巨噬细胞中。经过两步色谱纯化后获得的具有抗炎特性的级分 Cb (C2) 和 Pp (P2) 包含分子量范围为 1-3 kDa 的肽。与 LPS 治疗相比，活性组分 C2 和 P2 的 TNF-α 基因表达降低至 1.6 倍和 1 倍差异，而 IL6 表达分别降低至 30 倍和 40 倍差异。级分 C2 和 P2 对炎症蛋白（TNF-α、IL6、NFκB 和 p-IκB）的抑制证实了抗炎活性。