Abstract

A new coumestan named 7,5'-dihydroxy-4'-(3''-hydroxy-3''-methyl-trans-isobut-1''-enyl) coumestan (1), together with five known compounds (2–6), was isolated from the EtOAc-soluble extract of the stems of Acanthopanax senticosus. Their structures were elucidated based on extensive spectroscopic analyses. All the isolates were evaluated for in vitro cytotoxic activities against four human cancer cells including HepG2, A549, HeLa and MCF-7. Among them, the new compound 1 was found to exhibit significant cytotoxic activity on HeLa cells with IC₅₀ value of 6.5 μM.

中文翻译：

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刺五加茎的化学成分及其细胞毒活性

摘要

一种名为 7,5'-dihydroxy-4'-(3''-hydroxy-3''- methyl- trans -isobut-1''-enyl) coumestan 的新香豆素 ( 1 )，以及五种已知化合物 ( 2- 6 )，从刺五加茎的 EtOAc 可溶性提取物中分离得到。基于广泛的光谱分析阐明了它们的结构。评估了所有分离物对包括 HepG2、A549、HeLa 和 MCF-7 在内的四种人类癌细胞的体外细胞毒活性。其中，新化合物1对HeLa细胞具有显着的细胞毒活性，IC ₅₀值为6.5 μM。