Abstract

The present study aimed to investigate the protective role of capsaicin in a rat model of 2,3,7,8-tetracholorodibenzo-p-dioxin (TCDD)-induced toxicity. Exposure to TCDD which is an environmental toxicant causes severe toxic effects in the animal and human tissues. Therefore, the potential protective effect of capsaicin in TCDD-induced organ damage was investigated in rats by measuring thiobarbituric acid reactive substances (TBARS) level, superoxide dismutase (SOD) activity, and glutathione (GSH) level in the heart, liver, and kidney tissues for oxidant/antioxidant balance. Thirty-two healthy adults (250–300 g weight and 3–4 months old) male Wistar albino rats were randomly distributed into four equal groups (n = 8): Control, CAP, TCDD, TCDD + CAP. A dose of 2 μg/kg TCDD or a dose of 25 mg/kg capsaicin were dissolved in corn oil and orally administered to the rats for 30 days. The results indicated that TCDD-induced oxidative stress by increasing the level of TBARS and by decreasing the levels of GSH, and SOD activity in the tissues of rats. However, capsaicin treatment was significantly decreased TBARS levels and was significantly increased GSH level and SOD activity (p < 0.05). In addition, capsaicin (25 mg/kg) significantly attenuated TCDD-induced histopathological alteration associated with oxidative stress in the heart, liver, and kidney tissues (p < 0.05). As capsaicin regulates oxidative imbalance and attenuates histopathological alterations in the rat tissues, it may be preventing agents in TCDD toxicity.

摘要

本研究旨在研究辣椒素在 2,3,7,8-四氯二苯并对二恶英 (TCDD) 诱导的毒性大鼠模型中的保护作用。接触作为环境毒物的 TCDD 会对动物和人体组织造成严重的毒性影响。因此，通过测量大鼠心脏、肝脏和肾脏中的硫代巴比妥酸反应物质 (TBARS) 水平、超氧化物歧化酶 (SOD) 活性和谷胱甘肽 (GSH) 水平，研究辣椒素对 TCDD 诱导的器官损伤的潜在保护作用。氧化/抗氧化平衡的组织。将 32 只健康成人（体重 250-300 g，3-4 个月大）雄性 Wistar 白化大鼠随机分为四个相等的组（n = 8)：控制、CAP、TCDD、TCDD + CAP。将 2 μg/kg TCDD 或 25 mg/kg 辣椒素溶解在玉米油中，给大鼠口服给药 30 天。结果表明，TCDD通过增加大鼠组织中TBARS水平和降低GSH水平和SOD活性来诱导氧化应激。然而，辣椒素处理显着降低了TBARS水平，显着提高了GSH水平和SOD活性（p  < 0.05）。此外，辣椒素 (25 mg/kg) 显着减弱 TCDD 诱导的与心脏、肝脏和肾脏组织中的氧化应激相关的组织病理学改变 ( p < 0.05)。由于辣椒素调节氧化失衡并减弱大鼠组织的组织病理学改变，它可能是预防 TCDD 毒性的药物。