ABSTRACT

Bare and functionalised haematite nanoparticles (NPs) are synthesised through the co-precipitation method and characterised using XRD, SEM, TEM, EDX, FTIR, and VSM. XRD confirmed the rhombohedral-based hexagonal structure of Fe₂O₃, while, TEM and SEM showed the formation of lamellar-shaped NPs with the grain size varied from 22 to 80 nm. VSM presented soft ferromagnetic behaviour. From in vivo evaluations, changes in serum levels are analysed at different days of study after administration of bare and coated NPs through the intraperitoneal route of administration in albino rats. Toxicity parameters revealed a transient increase at day 1 but a gradual decrease thereafter. For biodegradation studies, NPs exposure in lysosomal-like medium showed insignificant changes in the absorbance spectrum for a short interval of time, however, dynamical degradation effects were observed on Day 8. Apoferritin studies disclose the growth of absorbance near 260 nm, which reveals progressive and partially filled proteins with iron ions.

摘要

裸露和功能化的赤铁矿纳米粒子 (NP) 通过共沉淀法合成，并使用 XRD、SEM、TEM、EDX、FTIR 和 VSM 进行表征。XRD 证实了 Fe ₂ O ₃的菱形六方结构，而 TEM 和 SEM 显示形成层状纳米颗粒，颗粒尺寸从 22 到 80 nm。VSM 呈现软铁磁行为。从体内根据评估，在白化大鼠中通过腹膜内给药途径给药裸露和包被的 NPs 后，在研究的不同天数分析血清水平的变化。毒性参数显示在第 1 天短暂增加，但此后逐渐减少。对于生物降解研究，NPs 在溶酶体样介质中的暴露在短时间内显示出吸光度光谱的微不足道变化，但是，在第 8 天观察到了动态降解效应。脱铁铁蛋白研究揭示了 260 nm 附近吸光度的增长，这表明吸光度是渐进的并用铁离子部分填充蛋白质。