Myoblasts are myogenic precursors that develop into myotubes during muscle formation. Improving efficiency of myoblast differentiation is important for advancing meat production by domestic animals. We recently identified novel oligodeoxynucleotides (ODNs) termed myogenetic ODNs (myoDNs) that promote the differentiation of mammalian myoblasts. An isoquinoline alkaloid, berberine, forms a complex with one of the myoDNs, iSN04, and enhances its activities. This study investigated the effects of myoDNs on chicken myoblasts to elucidate their species-specific actions. Seven myoDNs (iSN01–iSN07) were found to facilitate the differentiation of chicken myoblasts into myosin heavy chain (MHC)-positive myotubes. The iSN04–berberine complex exhibited a higher myogenetic activity than iSN04 alone, which was shown to enhance the differentiation of myoblasts into myotubes and the upregulation of myogenic gene expression (MyoD, myogenin, MHC, and myomaker). These data indicate that myoDNs promoting chicken myoblast differentiation may be used as potential feed additives in broiler diets.

中文翻译：

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黄连素复合成肌寡核苷酸促进鸡成肌细胞分化

成肌细胞是在肌肉形成过程中发育成肌管的生肌前体。提高成肌细胞分化的效率对于提高家畜的肉产量很重要。我们最近发现了新的寡脱氧核苷酸 (ODN)，称为肌源性 ODN (myoDN)，可促进哺乳动物成肌细胞的分化。异喹啉生物碱小檗碱与其中一种 myoDNs iSN04 形成复合物，并增强其活性。本研究调查了 myoDN 对鸡成肌细胞的影响，以阐明它们的物种特异性作用。发现七个 myoDN (iSN01–iSN07) 促进鸡成肌细胞分化为肌球蛋白重链 (MHC) 阳性肌管。iSN04-小檗碱复合物表现出比单独的 iSN04 更高的肌生成活性，这被证明可以增强成肌细胞向肌管的分化和生肌基因表达（MyoD、肌细胞生成素、MHC 和肌生成器）的上调。这些数据表明，促进鸡成肌细胞分化的 myoDN 可用作肉鸡日粮中的潜在饲料添加剂。