ABSTRACT

The ribonucleoprotein RNase MRP is responsible for the processing of ribosomal RNA precursors. It is found in virtually all eukaryotes that have been examined. In the Euglenozoa, including the genera Euglena, Diplonema and kinetoplastids, MRP RNA and protein subunits have so far escaped detection using bioinformatic methods. However, we now demonstrate that the RNA component is widespread among the Euglenozoa and that these RNAs have secondary structures that conform to the structure of all other phylogenetic groups. In Euglena, we identified the same set of P/MRP protein subunits as in many other protists. However, we failed to identify any of these proteins in the kinetoplastids. This finding poses interesting questions regarding the structure and function of RNase MRP in these species.

摘要

核糖核蛋白 RNase MRP 负责处理核糖体 RNA 前体。它几乎存在于所有经过检查的真核生物中。在眼虫属中，包括眼虫属、Diplonema 属和动质体，迄今为止，MRP RNA 和蛋白质亚基未能使用生物信息学方法进行检测。然而，我们现在证明 RNA 成分在眼虫中广泛存在，并且这些 RNA 具有符合所有其他系统发育组结构的二级结构。在眼虫中，我们发现了与许多其他原生生物相同的一组 P/MRP 蛋白亚基。然而，我们未能在动质体中鉴定出任何这些蛋白质。这一发现对这些物种中 RNase MRP 的结构和功能提出了有趣的问题。