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QTL analysis for grazing tolerance, autumn dormancy and growth habit offers prospects for marker-assisted selection in lucerne
Euphytica ( IF 1.9 ) Pub Date : 2021-07-26 , DOI: 10.1007/s10681-021-02897-7
Luciano Pecetti 1 , Paolo Annicchiarico 1 , Philippe Barre 2 , Sabrina Delaunay 2 , Paola Lambroni 2 , Bernadette Julier 2
Affiliation  

Selection of grazing-tolerant lucerne (Medicago sativa) germplasm with low autumn dormancy and erect growth habit, suitable to environments with mild winters, is hindered by genetically-based tradeoffs among these traits. Marker-assisted selection (MAS) may help overcoming the difficulty of phenotypic selection and hasten the breeding process. Over 400 progenies from four bi-parental crosses between contrasting parents from two cultivars were phenotyped for grazing tolerance and six other morpho-physiological characters. In parallel, genotyping-by-sequencing marker data were generated, with the objective to facilitate the multi-trait MAS by identifying quantitative trait loci (QTL) for the traits and assessing the extent of their co-location. In total, 9594 markers, with less than 20% missing data, segregated as simplex markers in at least one progeny family. Twenty-seven QTL were identified for six traits in the different bi-parental crosses. QTL were found to a different extent on all homology groups except homology group 4. No QTL was found for dry-matter yield before grazing imposition. The number of QTL for other traits varied from one (basal plant diameter) to nine (plant growth habit). Only for growth habit were QTL observed in all four crosses. The QTL analysis largely confirmed the association between grazing tolerance, non-erect growth habit and autumn dormancy. However, the absence of complete co-location of QTL highlighted a partly different genetic control of these traits, thereby offering scope for MAS exploitation to select genotypes carrying useful trait combinations.



中文翻译:

放牧耐受性、秋季休眠和生长习性的 QTL 分析为苜蓿标记辅助选择提供了前景

耐放牧苜蓿(紫花苜蓿)的选择) 具有低秋季休眠和直立生长习性的种质,适合冬季温和的环境,受到这些性状之间基于遗传的权衡的阻碍。标记辅助选择 (MAS) 可能有助于克服表型选择的困难并加快育种过程。对来自两个品种的对比亲本之间的四个双亲杂交的 400 多个后代进行了放牧耐受性和其他六个形态生理特征的表型分析。同时,通过测序标记数据进行基因分型,目的是通过识别性状的数量性状基因座 (QTL) 并评估其共定位的程度来促进多性状 MAS。总共有 9594 个标记,缺失数据少于 20%,在至少一个后代家族中被分离为单一标记。在不同的双亲杂交中,针对六个性状鉴定了 27 个 QTL。除同源组4外,所有同源组均存在不同程度的QTL。未发现放牧前干物质产量的QTL。其他性状的 QTL 数量从 1(植物基础直径)到 9(植物生长习性)不等。在所有四个杂交中仅观察到生长习性的QTL。QTL分析在很大程度上证实了放牧耐受性、非直立生长习性和秋季休眠之间的关联。然而,QTL 完全共定位的缺乏突出了这些性状的部分不同的遗传控制,从而为 MAS 开发提供了选择携带有用性状组合的基因型的空间。除同源组4外,所有同源组均存在不同程度的QTL。未发现放牧前干物质产量的QTL。其他性状的 QTL 数量从 1(植物基础直径)到 9(植物生长习性)不等。在所有四个杂交中仅观察到生长习性的QTL。QTL分析在很大程度上证实了放牧耐受性、非直立生长习性和秋季休眠之间的关联。然而,QTL 完全共定位的缺乏突出了这些性状的部分不同的遗传控制,从而为 MAS 开发提供了选择携带有用性状组合的基因型的空间。除同源组4外,所有同源组均存在不同程度的QTL。未发现放牧前干物质产量的QTL。其他性状的 QTL 数量从 1(植物基础直径)到 9(植物生长习性)不等。在所有四个杂交中仅观察到生长习性的QTL。QTL分析在很大程度上证实了放牧耐受性、非直立生长习性和秋季休眠之间的关联。然而,QTL 完全共定位的缺乏突出了这些性状的部分不同的遗传控制,从而为 MAS 开发提供了选择携带有用性状组合的基因型的空间。在所有四个杂交中仅观察到生长习性的QTL。QTL分析在很大程度上证实了放牧耐受性、非直立生长习性和秋季休眠之间的关联。然而,QTL 完全共定位的缺乏突出了这些性状的部分不同的遗传控制,从而为 MAS 开发提供了选择携带有用性状组合的基因型的空间。在所有四个杂交中仅观察到生长习性的QTL。QTL分析在很大程度上证实了放牧耐受性、非直立生长习性和秋季休眠之间的关联。然而,QTL 完全共定位的缺乏突出了这些性状的部分不同的遗传控制,从而为 MAS 开发提供了选择携带有用性状组合的基因型的空间。

更新日期:2021-07-26
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