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Microfluidic recapitulation of circulating tumor cell–neutrophil clusters via double spiral channel-induced deterministic encapsulation
Lab on a Chip ( IF 6.1 ) Pub Date : 2021-07-15 , DOI: 10.1039/d1lc00433f
Junhyun Park 1 , Sunyoung Park 1 , Kyung A Hyun 1 , Hyo-Il Jung 1
Affiliation  

Circulating tumor cell (CTC)–neutrophil clusters are highly potent precursors of cancer metastasis. However, their rarity in patients' blood has restricted research thus far, and moreover, studies on in vitro methods for mimicking cell clusters have generally neglected in vivo conditions. Here, we introduce an inertial-force-assisted droplet microfluidic chip that allows the recapitulation of CTC–neutrophil clusters in terms of physical as well as biochemical features. The deterministic encapsulation of cells via double spiral channels facilitates the pairing of neutrophils and cancer cells with ratios of interest (from 1 : 1 to 1 : 3). The encapsulated cells are spontaneously associated to form clusters, achieving the physical emulation of CTC–neutrophil clusters. Furthermore, the molecular signatures of CTC–neutrophil clusters (e.g., their E-cadherin, VCAM-1, and mRNA expressions) were well defined. Our novel microfluidic platform for exploring CTC–neutrophil clusters can therefore play a promising role in cancer-metastasis studies.

中文翻译:

通过双螺旋通道诱导的确定性封装循环肿瘤细胞 - 中性粒细胞簇的微流体再现

循环肿瘤细胞 (CTC)-中性粒细胞簇是癌症转移的高效前体。然而,迄今为止,它们在患者血液中的稀有性限制了研究,而且,对模拟细胞簇的体外方法的研究普遍忽略了体内条件。在这里,我们介绍了一种惯性力辅助的液滴微流控芯片,它允许在物理和生化特征方面重现 CTC-中性粒细胞簇。细胞的确定性封装通过双螺旋通道促进嗜中性粒细胞和癌细胞以感兴趣的比率(从 1:1 到 1:3)配对。被包裹的细胞自发地结合形成簇,实现 CTC-中性粒细胞簇的物理模拟。此外,CTC-中性粒细胞簇的分子特征(例如,它们的 E-钙粘蛋白、VCAM-1 和 mRNA 表达)得到了很好的定义。因此,我们用于探索 CTC-中性粒细胞簇的新型微流体平台可以在癌症转移研究中发挥有希望的作用。
更新日期:2021-07-26
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