Conventional two-dimensional (2D) cell culture is dramatically different from three-dimensional (3D) in vivo environment cells experience in vivo. Here, a new kind of 3D cell culture with a biomimetic structure to natural extracellular matrix (ECM) based on bacterial cellulose (BC) nanofibers and poly(glycerol-sebacate) (PGS) was designed. The 3D PGS/BC cell culture shows a porous and nanofibrous structure which was formed through biosynthesis of nanofibers secreted by G. xylinus on the porous PGS. The mechanical properties of PGS were significantly enhanced by the incorporation of BC nanofibers, which improved sample handling for application. L929 mouse fibroblasts and HUVECs were used as model cells to evaluate the potential ability of PGS/BC as cell culture. The results show that 3D PGS/BC promotes cell growth by facilitating cell proliferation, cell morphology and exhibit comparable performance to commercial Alvetex® cell culture. The BC nanofibers reinforced PGS/BC cell culture provides a 3D biomimetic structure to ECM which is beneficial to cellular adhesion and interaction. It is believed that the degradable PGS/BC has great potential applications for cell culture or tissue engineering scaffold.

传统的二维 (2D) 细胞培养与细胞在体内体验的三维 (3D) 体内环境有很大不同。在这里，设计了一种基于细菌纤维素 (BC) 纳米纤维和聚甘油癸二酸酯 (PGS) 的具有仿生结构的天然细胞外基质 (ECM) 的新型 3D 细胞培养物。3D PGS/BC 细胞培养物显示出多孔和纳米纤维结构，这是通过G. xylinus分泌的纳米纤维的生物合成形成的在多孔 PGS 上。通过掺入 BC 纳米纤维，PGS 的机械性能得到显着增强，从而改善了应用中的样品处理。L929 小鼠成纤维细胞和 HUVECs 被用作模型细胞来评估 PGS/BC 作为细胞培养的潜在能力。结果表明，3D PGS/BC 通过促进细胞增殖和细胞形态来促进细胞生长，并表现出与商业 Alvetex® 细胞培养物相当的性能。BC 纳米纤维增强的 PGS/BC 细胞培养物为 ECM 提供了 3D 仿生结构，这有利于细胞粘附和相互作用。相信可降解的PGS/BC在细胞培养或组织工程支架方面具有巨大的潜在应用。