There are currently no vaccines or effective drugs to prevent the disorders caused by avian leukosis virus subgroup J (ALV-J). Hence, it is critical to identify potential biomarkers in ALV-J-infected chickens to prevent ALV-J-induced disorders. We hypothesized that ALV-J infection alters metabolic profile in chickens. In the present study, a nontargeted metabolomics approach based on liquid chromatography coupled with mass spectrometry (LC-MS) was used to find differential metabolites in plasma samples from ALV-J-infected chickens and healthy controls. The parametric statistical test (Student's t-test) and fold change analysis were used for univariate analysis. Multivariate statistical analyses included principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The levels of methyl bromide, pyraclonil, hexaflumuron, lythidathion, 3-phosphoglycerol-glutathione, bis-4-nitrophenyl phosphate, 4-ketocyclophosphamide, oxidized photinus luciferin, phenyl sulfate, and aryl sulfate significantly decreased, whereas the levels of 2-methylthiobenzothiazole, irinotecan, methadone, 3-o-ethyl-l-ascorbic acid, and o-acetylneuraminic acid markedly increased in ALV-J-infected chickens as compared to those in healthy controls. These data provide metabolic evidence and potential biomarkers for ALV-J-induced alterations in plasma metabolism.

目前尚无疫苗或有效药物预防禽白血病病毒J亚群（ALV-J）引起的疾病。因此，识别 ALV-J 感染鸡的潜在生物标志物以预防 ALV-J 诱导的疾病至关重要。我们假设 ALV-J 感染会改变鸡的代谢特征。在本研究中，基于液相色谱与质谱联用 (LC-MS) 的非靶向代谢组学方法用于发现来自 ALV-J 感染鸡和健康对照的血浆样品中的差异代谢物。参数统计检验（Student's t-test) 和倍数变化分析用于单变量分析。多变量统计分析包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）。溴甲烷、吡咯菌酮、六氟尿嘧啶、lythidathion、3-磷酸甘油-谷胱甘肽、磷酸双-4-硝基苯酯、4-酮环磷酰胺、氧化荧光素、苯硫酸盐和芳基硫酸盐的含量显着降低，而2-甲硫基苯并噻唑、与健康对照相比，ALV-J 感染鸡的伊立替康、美沙酮、3-o-乙基-l-抗坏血酸和o-乙酰神经氨酸显着增加。这些数据为 ALV-J 诱导的血浆代谢改变提供了代谢证据和潜在的生物标志物。