With the introduction of tandem mass spectrometry to inductively coupled plasma mass spectrometry (ICPMS/MS), the potential for non-targeted elemental metabolomic analysis has been expanded to many non-metals of pivotal biological importance. Arsenic and selenium are trace elements that share chemical similarity with the non-metals phosphorus and sulfur, respectively, and this similarity can be exploited to gain more insight into the incompletely understood biological significance of these metalloids and the evolution of their biochemical pathways. As a proof of concept, we show the applicability of HPLC-ICPMS/MS for non-targeted and parallel speciation analysis of arsenic, selenium, phosphorus, and sulfur in mushrooms-metabolically diverse organisms. Incredibly contrasting levels of diversity were found in the metabolomic profiles of the four investigated elements among the various species along with sharp discrepancies among related elements (e.g. phosphorous vs. arsenic) in certain mushroom species. The present work shows that ICPMS/MS offers a new dimension in non-targeted metabolomic analysis and enables a unique comparative approach in investigating and tracking the biochemistry of related elements in moderately complex organisms.

中文翻译：

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通过 HPLC-ICPMS/MS 对蘑菇中的类金属及其非金属类似物进行平行和比较的非靶向代谢组学形态分析。

随着串联质谱法引入电感耦合等离子体质谱法 (ICPMS/MS)，非靶向元素代谢组学分析的潜力已扩展到许多具有关键生物学意义的非金属。砷和硒是微量元素，分别与非金属磷和硫具有化学相似性，可以利用这种相似性来更深入地了解这些非金属的生物学意义及其生化途径的演变。作为概念验证，我们展示了 HPLC-ICPMS/MS 对蘑菇代谢多样性生物体中砷、硒、磷和硫的非靶向和平行形态分析的适用性。在不同物种中四种被调查元素的代谢组学特征中发现了令人难以置信的差异水平，以及某些蘑菇物种中相关元素（例如磷与砷）之间的明显差异。目前的工作表明，ICPMS/MS 为非靶向代谢组学分析提供了一个新维度，并为调查和跟踪中等复杂生物体中相关元素的生化提供了一种独特的比较方法。