Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

中文翻译：

---

多光谱活体显微镜，可同时对微血管进行明场和荧光成像

活体视频显微镜可以观察微循环血流。这通常需要荧光探针来可视化传统明场显微镜无法观察到的结构和动态过程。传统的光学显微镜不允许同时进行明场和荧光成像。此外，在传统显微镜中，只能观察到一种类型的荧光标记。本研究介绍了多光谱活体视频显微镜，它在标准光学显微镜中结合了明视野和荧光显微镜。该技术能够同时实时观察与其直接物理环境相关的荧光标记结构。这一进步为微循环中标记结构的方向、运动和功能提供了背景。