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Using office inkjet printer to develop paper-based electrowetting-on-dielectric micromixer based on capillary wave-induced droplet vibration mixing for the reproducibility improvement of chemiluminescence assays
Journal of the Taiwan Institute of Chemical Engineers ( IF 5.5 ) Pub Date : 2021-07-24 , DOI: 10.1016/j.jtice.2021.07.014
Yuan-Yu Chen, I-Jung Ting, Shau-Chun Wang

Background

We use inkjet printing and film adhesion methods to economically develop paper-based electrowetting-on-dielectric (EWOD) mixing device. Because the periodic shrinking and stretching of droplet contact line induces surface capillary wave, the coalesced droplet on EWOD device vibrates to accelerate solute mixing on the merged spot.

Methods

An office inkjet printer fabricates pads of conducting silver nanoparticles to compose EWOD activation electrodes on a piece of glossy label paper, covered with one oil-coated dielectric film. Using the triggering relays of each pad, two droplets of thirty microliter individually loaded on the electrode ends are driven to coalesce. Alloy nanorod-catalyzed chemiluminescence assay can be performed on this mixing device.

Findings

When ac voltage of 100 Hz is applied on the electrode flakes, compared with solute transport efficiency in static condition, solute diffusivity is enhanced by 4 times using this mixing device. Linear relation (R2 = 0.99) between luminescence intensity acquired with smartphone camera and luminol concentration of aforementioned chemiluminescence assay is obtained, demonstrating reproducibility improvement for accurate determination.



中文翻译:

使用办公喷墨打印机开发基于毛细管波诱导液滴振动混合的纸基电润湿介质微混合器,以提高化学发光测定的重现性

背景

我们使用喷墨印刷和薄膜粘合方法来经济地开发纸基电介质电润湿 (EWOD) 混合设备。由于液滴接触线的周期性收缩和拉伸引起表面毛细管波,EWOD 装置上的聚结液滴振动以加速融合点上的溶质混合。

方法

办公室喷墨打印机在一张光滑的标签纸上制造导电银纳米粒子垫,以组成 EWOD 激活电极,标签纸覆盖着一层油涂层介电膜。使用每个垫的触发继电器,驱动电极端上分别装载的 30 微升的两个液滴聚结。合金纳米棒催化的化学发光分析可以在这个混合装置上进行。

发现

当在电极片上施加 100 Hz 的交流电压时,与静态条件下的溶质传输效率相比,使用该混合装置将溶质扩散率提高了 4 倍。 获得了智能手机相机获得的发光强度与上述化学发光测定的鲁米诺浓度之间的线性关系(R 2 = 0.99),证明了准确测定的重现性改进。

更新日期:2021-08-29
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