Traumatic brain injury (TBI) is one of the significant causes of death among young people worldwide. Doxycycline (DOX), an antibiotic with anti-inflammatory effects, has not been used as a therapeutic agent to modify the inflammatory response after the traumatic brain injury. In this study, intraperitoneal administration of DOX reduced significantly the acute inflammatory markers like IL-6 and CD3, microglial migration to the damaged area marked with Iba-1, and neuronal apoptosis assessed with TUNEL assay at 72 h after the trauma. The low dose, 10 mg/kg of DOX had a dominant anti-inflammatory effect; while the high dose, 100 mg/kg of DOX, was more effective in decreasing neuronal apoptosis. In early hours after the head trauma, use of a low dose (10 mg/kg) of DOX for decreasing the acute form of inflammation followed by a high dose (100 mg/kg) for the anti-apoptotic effects particularly in severe head traumas, would be a promising approach to alleviate the brain injury.

创伤性脑损伤 (TBI) 是全球年轻人死亡的重要原因之一。强力霉素 (DOX) 是一种具有抗炎作用的抗生素，尚未用作治疗外伤性脑损伤后炎症反应的治疗剂。在这项研究中，腹腔注射 DOX 显着降低了急性炎症标志物，如 IL-6 和 CD3，小胶质细胞向用 Iba-1 标记的受损区域的迁移，以及在创伤后 72 小时用 TUNEL 测定评估的神经元凋亡。低剂量的 DOX 10 mg/kg 具有显着的抗炎作用；而高剂量，100 毫克/公斤的 DOX，在减少神经元凋亡方面更有效。在头部外伤后的早期，