The relevance of the endocrine condition in microglia morphology and dendrite complexity of doublecortin-associated neurons in young adult and middle-aged female rats exposed to acute stress,European Journal of Neuroscience

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The relevance of the endocrine condition in microglia morphology and dendrite complexity of doublecortin-associated neurons in young adult and middle-aged female rats exposed to acute stress
European Journal of Neuroscience ( IF 2.7 ) Pub Date : 2021-07-23 , DOI: 10.1111/ejn.15398
Nelly Maritza Vega‐Rivera ₁ , Edgar González‐Monroy ₁ , Erik Morales Santana ₂ , Erika Estrada‐Camarena ₁

Affiliation

Menopause, natural or surgical, might facilitate the onset of psychiatric pathologies. Some reports suggest that their severity could increase if the decline of ovarian hormones occurs abruptly and before natural endocrine senescence. Therefore, we compared the effects of ovariectomy on microglia's morphological alterations, the complexity of newborn neurons, and the animal's ability to cope with stress. Young adult (3 months) and middle-aged (15 months) female Wistar rats were subjected to an ovariectomy (OVX) or were sham-operated. After 3 weeks, animals were assigned to one of the following independent groups: (1) young adult OVX + no stress; (2) young adult sham + no stress; (3) young adult OVX + stress; (4) young adult sham + stress; (5) middle-aged OVX + no stress; (6) middle-aged sham + no stress; (7) middle-aged OVX + stress; (8) middle-aged sham + stress. Acute stress was induced by forced swimming test (FST) exposure. Immobility behavior was scored during FST and 30 min after; animals were euthanized, their brains collected and prepared for immunohistochemical detection of Iba-1 to analyze morphological alterations in microglia, and doublecortin (DCX) detection to evaluate the dendrite complexity of newborn neurons. OVX increased immobility behavior, induced microglia morphological alterations, and reduced dendrite complexity of newborn neurons in young adult rats. FST further increased this effect. In middle-aged rats, the main effects were related to the aging process without OVX or stress exposure. In conclusion, surgical menopause favors in young adult rats, but not in middle-aged, the vulnerability to develop immobility behavior, retracted morphology of microglial cells, and decreased dendrite complexity of newborn neurons.

中文翻译：

暴露于急性应激的年轻和中年雌性大鼠小胶质细胞形态和双皮质素相关神经元树突复杂性的内分泌条件的相关性

更年期，自然的或手术的，可能会促进精神疾病的发作。一些报告表明，如果卵巢激素的下降在自然内分泌衰老之前突然发生，它们的严重程度可能会增加。因此，我们比较了卵巢切除术对小胶质细胞形态学改变、新生神经元复杂性和动物应对压力的能力的影响。年轻成年（3 个月）和中年（15 个月）雌性 Wistar 大鼠接受卵巢切除术 (OVX) 或假手术。3 周后，将动物分配到以下独立组之一：(1) 年轻成人 OVX + 无压力；(2) 青年假+无压力；(3) 青壮年OVX+压力；(4) 年轻成人假装 + 压力；（5）中年OVX+无压力；（6）中年假+无压力；(7)中年OVX+压力；(8)中年假装+压力。强迫游泳试验（FST）暴露诱发急性应激。在 FST 期间和 30 分钟后对不动行为进行评分；对动物进行安乐死，收集大脑并准备进行 Iba-1 免疫组化检测以分析小胶质细胞的形态学改变，以及双皮质素 (DCX) 检测以评估新生神经元的树突复杂性。OVX 增加了不动行为，诱导了小胶质细胞形态学改变，并降低了年轻成年大鼠新生神经元的树突复杂性。FST 进一步增加了这种影响。在中年大鼠中，主要影响与没有 OVX 或压力暴露的衰老过程有关。总之，手术更年期有利于年轻的成年大鼠，但不利于中年大鼠发展不动行为的脆弱性，

更新日期：2021-08-17

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