Abstract

Thermal springs have been the most resourceful ecological niches to understand the intricacies of the microbial community structure building. In the present study, the microbial community structure was investigated in five ecologically different hot springs. The highest number of OTUs was observed at low temperatures (42 °C) whereas an increasing number of unclassified bacteria was observed with a temperature rise. The statistical correlation predicted that temperature, total dissolved solids and ions were the primary environmental factors in controlling the community composition and diversity. The relative abundance of Proteobacteria, Cyanobacteria showed a positive correlation with moderate temperature whereas growth of Chloroflexi and Nitrospira was unstable at 65 °C. The observed LCBD was negatively correlated to the bacteria richness. A high relative abundance of Planctomycetes was restricted to Odisha hot springs (AT, TP, and DJ). We further hypothesize that abundance of most common cellulose-degrading bacteria such as Acinetobacter, Pseudomonas and Aeromonas only in DJ hot spring is possibly due to the high carbon content of the runoff water received from dense pandanus forest around it and could be a prospective source of industrially relevant cellulase after detailed characterization. The present study concludes that the association of physicochemical components with key species drive the microbial community structure.

摘要

温泉一直是了解微生物群落结构构建复杂性的最丰富的生态位。在本研究中，研究了五个生态不同的温泉中的微生物群落结构。在低温 (42 °C) 下观察到的 OTU 数量最多，而随着温度升高，未分类细菌的数量增加。统计相关性预测温度、总溶解固体和离子是控制群落组成和多样性的主要环境因素。Proteobacteria、Cyanobacteria的相对丰度与中等温度呈正相关，而Chloroflexi和Nitrospira在 65 °C 时不稳定。观察到的 LCBD 与细菌丰富度呈负相关。相对丰度较高的浮游菌仅限于奥里萨邦的温泉（AT、TP 和 DJ）。我们进一步假设，最常见的纤维素降解细菌如丰不动杆菌，假单胞菌和嗜只有在DJ温泉可能是由于来自周围茂密的露兜树森林收到的径流水的碳含量高，并可能是一个潜在来源详细表征后的工业相关纤维素酶。本研究得出结论，物理化学成分与关键物种的关联驱动了微生物群落结构。