The anthracnose pathogen Colletotrichum sublineola and its sorghum host provide an excellent system to examine concepts associated with gene-for-gene resistance. In this study, an excised-leaf spot inoculation assay method was used to evaluate host-pathogen interactions across several sorghum cultivars that serve as host differentials for defining races of the pathogen. Host cultivars tested included sorghum cultivars BTx398, RTx2536, Theis and johnsongrass cultivar SH1152. The cultivars were inoculated at varying conidia concentrations both singly and in pairs with conidia from strains of C. sublineola that differ in ability to incite the disease anthracnose. The hypothesis tested was that host defense triggered by effectors produced by an avirulent strain will limit infection when simultaneously inoculated with a strain that otherwise would lead to disease. Observations revealed significant changes in disease ratings based on conidial concentrations of the C. sublineola isolates used. However, disease rating scores for combined inocula in the excised-leaf spot inoculation assays led to scores either between the scores generated by single isolates or near the score of the more virulent isolate.

炭疽病病原体Colletotrichum sublineola及其高粱宿主提供了一个极好的系统来检查与基因对基因抗性相关的概念。在这项研究中，使用离体叶点接种试验方法来评估几个高粱品种之间的宿主-病原体相互作用，这些高粱品种作为宿主差异，用于定义病原体的种族。测试的宿主品种包括高粱品种 BTx398、RTx2536、Theis 和 johnsongrass 品种 SH1152。栽培品种以不同的分生孢子浓度单独或成对接种来自C. sublineola菌株的分生孢子导致炭疽病的能力不同。测试的假设是，当同时接种会导致疾病的菌株时，由无毒菌株产生的效应物触发的宿主防御将限制感染。观察结果显示，基于所使用的C. sublineola分离株的分生孢子浓度，疾病等级发生了显着变化。然而，在离体叶斑点接种试验中组合接种物的疾病等级评分导致评分介于单个分离株产生的评分之间或接近毒性更强的分离株的评分。