Probes for in vivo imaging of hypochlorous acid (HOCl), one of the most important reactive oxygen species in innate immunity, are urgently needed to understand the pathogenesis of autoimmune and neuroinflammatory disorders. As a strong oxidant, HOCl could bleach near-infrared sensors and inactivate luciferase readily, making in vivo imaging overwhelmingly challenging. Via fine-tuning of a selective HOCl sensing moiety, HOCl stable spacer, and bright chemiluminescent scaffold, we have developed HOCl-CL-510 as a highly selective and sensitive probe for HOCl detection both in vitro and in vivo. In particular, we achieved recurring real-time monitoring of HOCl in both acute and chronic inflammation models in living mice, providing a new chemical tool for dynamic monitoring of disease development with reduced usage of experimental animals.

次氯酸 (HOCl) 是先天免疫中最重要的活性氧之一，迫切需要用于体内成像的探针，以了解自身免疫和神经炎症性疾病的发病机制。作为一种强氧化剂，HOCl 可以漂白近红外传感器并容易地灭活荧光素酶，这使得体内成像极具挑战性。通过微调选择性 HOCl 传感部分、HOCl 稳定间隔物和明亮的化学发光支架，我们开发了 HOCl-CL-510作为用于体外和体内 HOCl 检测的高选择性和灵敏探针。特别是，我们在活体小鼠的急性和慢性炎症模型中实现了对 HOCl 的重复实时监测，为动态监测疾病发展提供了一种新的化学工具，减少了实验动物的使用。