Objectives

To produce flavonol and flavone 6-C-glucosides by bioconversion using recombinant Escherichia coli expressing a C-glucosyltransferase from wasabi (WjGT1).

Results

Escherichia coli expressing WjGT1 (Ec-WjGT1) converted flavones (apigenin and luteolin) and flavonols (quercetin and kaempferol) into their 6-C-glucosides in M9 minimal media supplemented with glucose, and released these products into the culture media. Ec-WjGT1 system also converts a flavanone (naringenin) into its C-glucoside at a conversion rate of 60% in 6 h. For scale-up production, apigenin, kaempferol, and quercetin were sequentially fed into the Ec-WjGT1 system at concentrations of 20–50 µM every 15–60 min, and the system was then able to produce isovitexin, kaempferol 6-C-glucoside, and quercetin 6-C-glucoside at an 89–99% conversion rate.

Conclusions

The Ec-WjGT1 system quickly and easily produces flavone and flavonol 6-C-glucosides at high conversion rates when using sequential administration to avoid precipitation of substrates.

中文翻译：

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在表达来自芥末（Eutrema japonicum）的 C-葡糖基转移酶的大肠杆菌中通过生物转化生产黄酮醇和黄酮 6-C-葡糖苷

目标

使用表达来自芥末的C-葡糖基转移酶 (WjGT1) 的重组大肠杆菌，通过生物转化生产黄酮醇和黄酮 6- C-葡糖苷。

结果

表达 WjGT1 (Ec-WjGT1) 的大肠杆菌在补充有葡萄糖的 M9 基本培养基中将黄酮（芹菜素和木犀草素）和黄酮醇（槲皮素和山柰酚）转化为它们的 6- C-葡萄糖苷，并将这些产物释放到培养基中。Ec-WjGT1 系统还在6 小时内以 60% 的转化率将黄烷酮（柚皮素）转化为其C-葡萄糖苷。为了扩大生产，将芹菜素、山柰酚和槲皮素以每 15-60 分钟 20-50 µM 的浓度依次加入 Ec-WjGT1 系统，然后该系统能够生产异牡荆素、山柰酚 6- C-葡萄糖苷和槲皮素 6- C-葡萄糖苷，转化率为 89-99%。

结论

当使用顺序给药避免底物沉淀时，Ec-WjGT1 系统可快速轻松地以高转化率生产黄酮和黄酮醇 6- C-葡糖苷。