Micro-arc oxidation (MAO) was used to improve the resistance of pure magnesium (Mg). Copper (Cu), a good antibacterial, angiogenic, and osteogenic element, was added by reaction in a Cu-containing electrolyte to improve the osteogenic and pro-angiogenic activities of Mg. The surface microstructures of the resulting MAO were evaluated by a scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) mapping. The release of Cu ions was detected by ICP-OES. The antibacterial activity of films with different concentrations of Cu ions was assessed against Staphylococcus aureus (S. aureus). The osteogenesis of films was confirmed by cell morphology and proliferation, ALP activity, alizarin red staining, and osteogenic-related gene expression in the MC3T3-E1 cell line. The angiogenesis of the films was tested in human umbilical vein endothelial cells (HUVECs) by cell migration, tube formation, and VEGF quantification in vitro, and by a chicken embryo chorioallantoic membrane (CAM) assay in vivo. The results showed that the microporous structure was shaped by MAO, and the Cu group was denser and more uniform. The Cu coating showed effective antibacterial activity against S. aureus while also enhancing osteogenesis and angiogenesis in vitro. According to the CAM assay, the Cu group showed not only biocompatibility but also a significant angiogenic response, which was consistent with in vitro studies. The findings indicate that a Cu coating on Mg-MAO enhances osteogenesis and angiogenesis.

微弧氧化（MAO）用于提高纯镁（Mg）的电阻。铜 (Cu) 是一种良好的抗菌、血管生成和成骨元素，通过在含铜电解质中反应添加，以提高 Mg 的成骨和促血管生成活性。通过扫描电子显微镜 (SEM) 和能量色散 X 射线光谱 (EDS) 映射评估所得 MAO 的表面微结构。Cu 离子的释放通过 ICP-OES 检测。评估了具有不同浓度 Cu 离子的薄膜对金黄色葡萄球菌( S. aureus)的抗菌活性。通过细胞形态和增殖、ALP 活性、茜素红染色和 MC3T3-E1 细胞系中的成骨相关基因表达证实了薄膜的成骨作用。通过体外细胞迁移、管形成和 VEGF 定量以及体内鸡胚绒毛膜尿囊膜 (CAM) 测定，在人脐静脉内皮细胞 (HUVEC) 中测试了薄膜的血管生成。结果表明微孔结构由MAO形成，Cu基团更致密、更均匀。铜涂层对金黄色葡萄球菌显示出有效的抗菌活性，同时还能增强体外的成骨和血管生成. 根据 CAM 测定，Cu 组不仅显示出生物相容性，而且显示出显着的血管生成反应，这与体外研究一致。研究结果表明，Mg-MAO 上的 Cu 涂层增强了成骨和血管生成。