The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (2G ethanol) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugar composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y’_P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for Y_P/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, Q_P was lower for MSC with immobilized cells, reaching 0.81 ± 0.04 g/L.h in the second batch, while for free cells the Q_P varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reused through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

在模拟用稀硫酸预处理的甘蔗渣半纤维素水解产物的培养基中，评估了将S. passalidarum固定在海藻酸钙珠中用于第二代乙醇生产（2G 乙醇）的糖成分。用高 (HSC) 和中等 (MSC) 初始糖浓度（分别为 120 和 70 g/L）的游离细胞或固定细胞进行三组连续分批发酵 (SBF)。SBF 的特点是在振荡器中连续进行五批，温度为 30 °C 和 110 rpm。在第二批循环中，与 HSC（Y' _{P/S 分别}为 0.27 ± 0.02 和 0.19 ± 0.03 g/g）相比，在 MSC 培养基中固定化细胞的 SBF 观察到了更好的结果。Y _P/S的值在 MSC 中的浓度与用游离细胞获得的相似（0.30 ± 0.02 至 0.33 ± 0.02 g/g）。然而，带有固定细胞的 MSC 的Q _P较低，在第二批中达到 0.81 ± 0.04 g/Lh，而对于游离细胞，Q _P从 1.06 ± 0.02 到 1.16 ± 0.22 g/Lh 变化。应用藻酸盐珠中的固定细胞，这使得确定所执行的 SBF 的特定速率成为可能。根据获得的结果，可以证明S. passalidarum可以固定在海藻酸钙中并通过 SBF 重复使用，性能与游离细胞相似，这可能是半纤维素水解物发酵的良好策略。