Abstract

Glyphosate, widely used herbicide, is considered harmful to the environment and human health. Its effects on growth rate and genotoxicity of Ipomoea aquatica were assessed in this work. The plants were grown in the soil which supplemented with glyphosate at 1- to 3-fold the recommended dose. After 30 days, lengths and dry weights of the roots and shoots were found to decrease in a concentration-dependent manner. The same was found for genotoxicity assessed via random amplified polymorphic DNA with genomic template stability tests, confirming the effects of glyphosate on the growth rate inhibition and DNA damage. The usefulness of the random amplified polymorphic DNA assay as ecotoxicological tool for assessing genotoxic effects in plants is demonstrated.

摘要

草甘膦是一种广泛使用的除草剂，被认为对环境和人类健康有害。在这项工作中评估了它对水生番薯的生长速度和遗传毒性的影响。植物在土壤中生长，土壤中添加了推荐剂量的 1 到 3 倍的草甘膦。30 天后，发现根和芽的长度和干重以浓度依赖性方式减少。通过随机扩增多态性 DNA 和基因组模板稳定性测试评估的遗传毒性也发现了同样的情况，证实了草甘膦对生长速率抑制和 DNA 损伤的影响。证明了随机扩增多态性 DNA 检测作为评估植物遗传毒性作用的生态毒理学工具的有用性。