Abstract

By using a bifunctional organic ligand 2-(benzimidazol-1-yl)terephthalic acid (H₂bitp) featuring both imidazol-carboxylate groups, two new Co(II)-containing coordination polymers (CPs) with the chemical formulae of {[Co(bitp)(bpa)_0.5(H₂O)₂]·0.5bpa}_n (1, bpa = 1,2-bis(4-pyridyl)ethylane) and {[Co_1.5(bitp)(Hbitp)(2,2′-bpy)(H₂O)]·3H₂O}_n (2, 2,2′-bpy = 2,2′-bipyridine) have been successfully prepared by reaction of Co(NO₃)₂·6H₂O with the H₂bitp ligand in the presence of different N-donor co-ligands. Their treatment activity on periodontitis was evaluated and the specific mechanism was then explored. After treated with compounds 1 and 2, the relative expression of the inflammatory genes nf-κb and p53 in periodontal mucosal cells was measured with real time RT-PCR (reverse transcription-polymerase chain reaction). ELISA (enzyme linked immunosorbent assay) detection kit was used to detect the release of the inflammatory cytokines in the gingival crevicular fluid after compounds 1 and 2 treatment. Compound 1 showed stronger biological activity than compound 2 on periodontitis treatment.

摘要

通过使用具有两个咪唑-羧酸酯基团的双功能有机配体 2-(苯并咪唑-1-基)对苯二甲酸 (H ₂ bitp)，两种新的含 Co(II) 的配位聚合物 (CP) 的化学式为 {[Co (bitp)(bpa) _0.5 (H ₂ O) ₂ ]·0.5bpa} _n ( 1 , bpa = 1,2-双(4-吡啶基)乙烷)和{[Co _1.5 (bitp)(Hbitp)(2, 2'-bpy)(H ₂ O)]·3H ₂ O} _n ( 2 , 2,2'-bpy = 2,2'-联吡啶) 由Co(NO ₃ ) ₂ ·6H ₂反应成功制备O 与 H ₂在不同 N 供体共配体存在下的 bitp 配体。评估了它们对牙周炎的治疗活性，然后探讨了具体机制。用化合物1和2处理后，用实时RT-PCR(逆转录-聚合酶链反应)测量牙周粘膜细胞中炎症基因nf-κb和p53的相对表达。采用ELISA（酶联免疫吸附试验）检测试剂盒检测化合物1和2处理后龈沟液中炎性细胞因子的释放情况。化合物1比化合物2表现出更强的生物活性 关于牙周炎的治疗。